抗白血病McAb HI30免疫脂质体的制备及其体外导向实验  

The Preparation of Monoclonal Antibody HI30- Homoharringtonine Liposomes (Immunoliposomes) and Targeting Tests In Viiro

作　　者：陈正秋[1] 沈德诚[1] 杨秀芬[1] 李树梅[1] 马玉芬 范启修[1] 

机构地区：[1]中国医学科学院血液学研究所

出　　处：《中国医学科学院学报》1991年第5期323-326,共4页Acta Academiae Medicinae Sinicae

摘　　要：以人红细胞膜脂为主要膜材,制备成功MPB高三尖杉酯碱脂质体(MPB-homoharri-ngtonine-liposomes,MPB-HH-Lip),再与鼠抗人白细胞McAb HI30共价偶联,经凝胶柱层析分离,得到McAb HI30高三尖杉酯碱脂质体(简称免疫脂质体,HI30-HH-Lip)。间接免疫荧光法检测表明,HI30-HH-Lip对人系靶细胞CEM-M3具有敏感的靶向作用,并基本上保持了McAb HI30的免疫活性。Liposomes encapsulating homoharringtonine were prepared using soybean phos-pholipids, egg yolk phospholipids and phospholipids from human red cell membrane, respectively. The quantity of homoharringtonine encapsulated by phospholipids from human red cell membrane was greater than that encapsulated by the other two types of liposomes, so this kind of phospholipid was used for further studies. The liposomes containing homoharringtonine (HH) were covalently coupled with HI30(CD45).The mixture was separated by gel column chromatography. HI30-HH -liposomes demonstrated a sensitive targeting function with an immune activity equal to that observed with original HI30 in indirect immunofluorescence tests using the human cell line CEM-M3.

关 键 词：单克隆抗体 免疫脂质体 靶向作用 

分 类 号：R392－33[医药卫生—免疫学]