长链非编码RNA LINC00460在喉鳞状细胞癌中的表达及意义  

Expression of long non-coding RNA LINC00460 in laryngeal squamous cell carcinoma tissue and its clinical significance

作　　者：葛珊珊[1] 吴勇延[1] 高伟[1] 张春明[1] 侯娟 温树信[1] 王斌全[1] 

机构地区：[1]山西医科大学第一医院耳鼻咽喉科,太原030001

出　　处：《临床耳鼻咽喉头颈外科杂志》2018年第1期18-22,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:明确长链非编码RNA LINC00460在喉鳞状细胞癌中的表达水平及临床意义。方法:利用实时荧光定量PCR技术检测68例喉鳞状细胞癌组织及配对的癌旁正常黏膜组织中LINC00460的表达水平,分析喉鳞状细胞癌TNM分期、颈淋巴结转移、病理类型、临床分期、分化程度等临床病理参数与LINC00460表达水平之间的关系。结果:喉鳞状细胞癌组织中LINC00460的表达显著高于癌旁正常黏膜组织(P=0.006)。结合临床病理参数分析,结果表明:LINC00460表达水平在声门上型、声门型、声门下型喉鳞状细胞癌中均差异无统计学意义(P>0.05);低分化喉鳞状细胞癌与高、中分化的喉鳞状细胞癌相比,LINC00460表达水平差异无统计学意义(P>0.05);无淋巴结转移喉鳞状细胞癌与有淋巴结转移喉鳞状细胞癌相比,LINC00460表达水平差异无统计学意义(P>0.05)。不同T分期喉鳞状细胞癌组织中,LINC00460在T1+T2期喉鳞状细胞癌中的表达水平显著低于T3+T4期(P<0.05)。结论:LINC00460可能作为重要的癌基因在喉鳞状细胞癌的发生、发展中发挥重要生物学功能。Objective：To understand the expression of LINC00460 in laryngeal squamous cell carcinoma（LSCC）,and to discuss the role of LINC00460 in carcinogenesis and development of laryngeal squamous cell carcinoma.Method：The expression of LINC00460 in 68 laryngeal squamous cell carcinoma tissues and paired adjacent normal tissues were examined by real-time PCR.The relationship between the expression levels of LINC00460 and the clinicopathological features including clinical stage,histological grade,pathological type,and lymph node metastasis in LSCC was analyzed according to the clinical data.Result：The expression of LINC00460 was significantly upregulated in the LSCC tissue compared with that of adjacent normal mucosal tissue（P=0.006）.There were no statistical differences of the quantity of LINC00460 expression among supraglottic,glottic and subglottic LSCC（P〈0.05）.Moreover,LINC00460 had no significant changes in poorly differentiated LSCC when compared with that of well and moderately differentiated LSCC（P〈0.05）.Furthermore,the expression of LINC00460 in LSCC with lymph node metastasis had no significant changes when compared with that without of lymph node metastasis（P〈0.05）.Notably,LINC00460 expression in T1＋T2 stages patients were significantly lower than T3＋T4 stages（P〈0.05）.Conclusion：Upregulation of long non-coding RNA LINC00460 might contribute to the carcinogenesis and development of LSCC,furthermore play important biological function.

关 键 词：长链非编码RNA 喉鳞状细胞癌 生物标志物 侵袭 转移 

分 类 号：R763.9[医药卫生—耳鼻咽喉科]