消定膏对骨折愈合过程中COX-2/PGE_2/cAMP信号通路表达的影响  

Effects of Xiaoding Ointment on COX-2/PGE_2/cAMP signal pathway expression in fracture healing

作　　者：董万涛 宋敏[2] 陈秉雄[3] 巩彦龙 刘涛 张洁[1] 刘保健 冯宜蒀 

机构地区：[1]甘肃中医药大学附属医院,甘肃兰州730020 [2]甘肃中医药大学,甘肃兰州730000 [3]临洮县人民医院,甘肃定西730500

出　　处：《中成药》2018年第1期20-26,共7页Chinese Traditional Patent Medicine

基　　金：甘肃省高校科研项目(2016B-056);吴阶平医学基金会临床科研专项基金资助项目(320.6750.11061);甘肃省青年自然科学基金计划(1208RJYA066)

摘　　要：目的研究消定膏(无名异、儿茶、大黄等)对兔环氧化酶-2(COX-2)、前列腺素E2(PGE2)、环磷酸腺苷(c AMP)不同时相表达的影响,探讨其对骨折愈合的作用机制。方法将128只新西兰大耳白兔随机分为空白组、模型组、青鹏膏组(棘豆、亚大黄、诃子等)和消定膏组,除空白组外,其余各组均采用骨缺损法造成尺骨3 mm骨缺损模型,造模成功后,空白组、模型组仅做外固定。用药后第3、7、14、28天各组随机抽取8只,取骨痂组织,光镜下观察病理形态学变化,实时荧光定量-聚合酶链式反应(qRT-PCR)检测骨痂组织COX-2 mRNA表达水平,免疫组化法检测骨痂组织PGE2、c AMP蛋白的表达。结果与模型组、青鹏膏组比较,消定膏组骨痂和胶原纤维的形成明显优于其他组,消定膏组COX-2 mRNA表达及PGE2、c AMP蛋白表达在术后7、14 d明显增强(P<0.05),且在术后14 d左右出现峰值。结论消定膏具有明显的促进骨折愈合作用,可能与其对COX-2/PGE2/c AMP信号通路的影响有关。AIM To study the effects and action mechnism of Xiaoding Ointment （Pyrolusite, Catechu, Rhei Radix et Rhizoma, etc. ） on rabbits＇ fracture healing in terms of eyelooxygenase-2 （COX-2）, prostaglandin E2 （PGE2 ）, cyclic adenosine monophosphate （cAMP） expressions at different time points. METHODS One hun- dred and twenty-eight New Zealand white rabbits were randomly divided into blank group, model group, Qingpeng Ointment （Oxytropis Falcatae Herba, Rhei lhasaense Radix et Rhizoma, Terminalia chebula Fructus, etc. ） group and Xiaoding Ointment group. All the other groups, except the blank group, were made with ulna 3 rmn bone defect, after which external fixation was applied to both the blank group and the model group. On the 3rd, 7th, 14th and 28th days after the medications, eight rabbits randomly selected from various groups had their callus morphopathology changes observed under optical microscope, their callus tissue COX-2 mRNA expression levels were detected by real- time fluorescence quantitative polymerase chain reaction （qRT-PCR）, and their callus tissue PGE2 and cAMP pro- tein expressions were determined by immunohistochemistry. RESULTS Compared with the model group and the Qingpeng Ointment group, the Xiaoding Ointment group exhibited significantly better formation of callus and collagen fibers. The mRNA expression of COX-2, and the protein expressions of PGE2 and cAMP in the Xiaoding Ointment group were remarkably enhanced as revealed on the 7th and 14th days＇ postoperative check （P 〈 0. 05 ）, and the peak values arrived around the 14th day after the operation. CONCLUSION Xiaoding Ointment＇ s obvious effect on promoting fracture healing may associate with its impact on COX-2/PGE2/cAMP signaling pathway.

关 键 词：消定膏 骨缺损 环氧化酶-2(COX-2) 前列腺素E2(PGE2) 环磷酸腺苷(cAMP) 

分 类 号：R285.5[医药卫生—中药学]