HPLC法同时测定五粒回春丸中8种成分  

Simultaneous determination of eight constituents in Wuli Huichun Pills by HPLC

作　　者：谭雄斯[1] 

机构地区：[1]肇庆医学高等专科学校,广东肇庆526020

出　　处：《中成药》2018年第1期110-113,共4页Chinese Traditional Patent Medicine

摘　　要：目的建立HPLC法同时测定五粒回春丸(防风、牛蒡子、连翘等)中牛蒡子苷、牛蒡子苷元、连翘酯苷A、柚皮芸香苷、柚皮苷、野漆树苷、升麻素苷、5-O-甲基维斯阿米醇苷的含有量。方法该药物70%甲醇提取液的分析采用Agilent TC-C18色谱柱(4.6 mm×250 mm,5μm);流动相甲醇-0.4%冰醋酸,梯度洗脱;体积流量0.9 m L/min;柱温25℃。结果 8种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.55%~99.50%,RSD0.54%~1.72%。结论该方法准确可靠,重复性好,可用于五粒回春丸的质量控制。AIM To establish an HPLC method for the simultaneous content determination of arctiin, aretige- nin, forsythoside A, narirutin, naringin, rhoifolin, prim-O-glucosylcimifugin and 5-O-methylvisammioside in Wuli Huichun Pills （Saposhnikoviae Radix, Arctii Fructus, Forsythiae Fructus, etc. ）. METHODS The analysis of 70% methanol extract of this drug was performed on a 25 ℃ thermostatic Agilent TC-C18 column （4.6 mm × 250 mm, 5 μm）, with the mobile phase comprising of methanol-0. 4% glacial acetic acid flowing at 0. 9 mL/min in a gradient elution manner. RESULTS Eight constituents showed good linear relationships within their own ranges （r 〉 0. 999 0） , whose average recoveries were 96.55% - 99.50% with the RSDs of 0. 54% - 1.72%. CONCLUSION This accurate, reliable and reproducible method can be used for the quality control of Wuli Huichun Pills.

关 键 词：五粒回春丸 化学成分 HPLC 

分 类 号：R927.2[医药卫生—药学]