超临界萃取海参脑苷脂及HPLC检测分析  

Supercritical Fluid Extraction and HPLC Detection Analysis of Cerebroside from Sea Cucumber

作　　者：徐志利[1] 赵雄伟[2] 孙剑锋[1] 刘茜[1] 孟淑静[1] 

机构地区：[1]河北农业大学食品科技学院,河北保定071000 [2]河北农业大学科技兴农中心,河北保定071000

出　　处：《食品研究与开发》2018年第2期46-50,共5页Food Research and Development

基　　金：河北省科技计划项目(14273205D);河北省高等学校科技技术研究项目(YQ2014037);河北省青年拔尖人才支持计划;河北省食品科学与工程学科"双一流"建设资金项目(2016SPGCA18)

摘　　要：通过超临界萃取的方式提取海参中的脑苷脂,利用单因素试验研究萃取温度、萃取时间、萃取压力以及海参粉的颗粒大小对超临界萃取海参中的脑苷脂提取率的影响,用正交试验来优化超临界萃取的条件,使用高效液相色谱(HPLC)来检测海参中脑苷脂的提取率。试验结果表明,适宜的超临界萃取条件是:萃取时间3 h,萃取温度38℃,萃取压力20 MPa,颗粒物大小60目,用高效液相色谱法分别测得了半乳糖脑苷脂的提取率,在前述测定条件下,海参中的脑苷脂最高提取率可达0.107 9%。By supercritical fluid extraction to extract cerebroside from sea cucumber ,and through the singlefactor experiment to explore the extraction temperature,extraction time,extraction pressure and sea cucumberpowder particle size on the cerebroside extraction yield of supercritical fluid extraction,using the orthogonal ex-periment to optimize the supercritical fluid extraction conditions,using high performance liquid chromatogra-phy（HPLC）to detect the sea cucumber cerebroside extraction yield. The experimental results showed that ap-propriate conditions of supercritical fluid extraction was extraction time 3h,extraction temperature 38 ℃,ex-traction pressure 20 MPa,the particle size 60 mesh. The extraction yield of galactose cerebroside was mea-sured by HPLC method. Under the determination conditions above, the highest extraction yield of cerebroside insea cucumber was 0.1079 %.

关 键 词：超临界萃取 高效液相色谱 脑苷脂 

分 类 号：O657.72[理学—分析化学] TS254.4[理学—化学]