响应曲面法优化茶树总黄酮提取工艺的研究  

Optimization of Extracting Technology for Total Flavonoids in Tea Tree with Response Surface Methodology

作　　者：秦文文 刘宇[1] 李妹妹 郝宝成[1] 王学红[1] 尚若锋[1] 黄鑫[1] 衣云鹏 梁剑平[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部兽用药物创制重点试验室甘肃省新兽药工程重点试验室,甘肃兰州730050

出　　处：《食品研究与开发》2018年第2期67-71,共5页Food Research and Development

基　　金：中国农业科学院科技创新工程兽用天然药物创新工程(CAAS-ASTIP-2014-LIHPS-04)

摘　　要：以广东茶树枝叶为原料,利用超声波辅助提取茶树总黄酮,并采用Box-Behnken对超声波超声时间、乙醇浓度、料液比进行响应面优化。结果表明:最佳提取工艺参数为乙醇浓度60%,料液比1∶30(g/m L),提取时间40 min。在此条件下,总黄酮的得率为29.7 mg/g,超声提取工艺合理、可行,为茶树总黄酮的进一步开发利用提供参考依据。Tea Tree from Guangdong province was used as the raw materials to extract total flavonoids. A novelapproach, ultrasonic-assisted method,for preparation of total flavonoids were developed. The optimal ultrasonic-assisted treatment was explored using response surface methodology. The results showed that the optimumextraction condition were ethanol concentration 60%,liquid ratio1 ： 30（g/mL） , extraction time 40 min. Underthe optimization conditions, the experimental yield was 29.7 mg/g. The method is reasonable and feasible, canprovide a theoretical reference for the development of new flavonoids in tea plant.

关 键 词：茶树 总黄酮 响应曲面法 

分 类 号：R284.2[医药卫生—中药学]