水蒸气蒸馏与超临界提取来凤凤菊成分的HS-GC/MS分析  

HS-GC/MS Analysis of the Extracts by Steam Distillation and Supercritical-CO_2 Fluid Extraction from Dendranthelna Indicum

作　　者：邢玉娟 陈新 刘梁 史茹茹 彭彩暾 

机构地区：[1]武汉轻工大学生物与制药工程学院,湖北武汉430023

出　　处：《食品研究与开发》2018年第2期149-154,共6页Food Research and Development

基　　金：国家自然科学基金项目(31370369);国家自然科学基金项目(21602166)

摘　　要：分析比较水蒸气蒸馏法与超临界CO_2萃取法提取来凤凤菊化学成分的差异。通过水蒸气蒸馏提取和超临界CO_2萃取法提取来凤凤菊中的化学成分,运用顶空气相色谱-质谱联用技术(headspace extraction-gas chromatography-mass spectrometry,HS-GC/MS)对其成分进行分离鉴定,用峰面积归一化法进行定量分析。水蒸气蒸馏提取法共分离出161种成分,鉴定出其中的56种,主要成分是:α-水芹烯(10.70%)、2,2,6-三甲基-6-乙烯基四氢-3-吡喃酮(7.14%)、芳樟醇(11.90%)、芳樟醇氧化物(7.95%)等;超临界CO2提取法共分离出121种成分,鉴定出其中的38种,主要成分是α-水芹烯(11.91%)、邻伞花烃(22.59%)、芳樟醇(6.95%)、芳樟醇氧化物(5.75%)等。两种不同的提取法共分离鉴定27种相同的化学成分,水蒸气蒸馏法多分离鉴定18种成分。To analyze the chemical constituent of the extracts from by differentextraction methods.The compounds were extracted by steam distillation and supercritical-CO2 fluid extraction.Constituents were separated and identified by headspace extraction -gas chromatography -mass spectrometry（HS -GC/MS）, and were quantified by the accuracy of area percent.161 components were separated amongwhich 56 were identified, the main components were alpha. -Phellandrene （10.70%）, Tetrahydro -2, 2, 6-trimethyl-6-vinyl-3--pyranone （7.14%）, Linalool （11.90%）, Linalool Oxide （7.95%） by steam distillation.121 components were separated among which 38 were identified,the main components were alpha.-Phellandrene （11.91%）, o -Cymene （22.59%）,Linalool （6.95%）, Linalool Oxide （5.75%）in thesupercritical-CO2 fluid extraction.27 chemical components of the extracts were the same by the two extractionmethods, but there are more than 18 components in the steam distillation.

关 键 词：HS-GC/MS 来凤凤菊 水蒸气蒸馏法 超临界CO2萃取法 

分 类 号：O657.63[理学—分析化学] TQ651.2[理学—化学]