基于UPLC-Q-TOF/MS技术的桑叶化学成分快速识别分析  

Fast Identification Analysis on Chemical Constituents in Mori Folium by Using UPLC-Q-TOF/MS Method

作　　者：刘金玲[1,2] 李文姣 王韧 李森 张雨恬[1,2] 周晋 贺福元 

机构地区：[1]湖南中医药大学药学院,湖南长沙410208 [2]中国中医药超分子机理与数理特征化实验室,湖南长沙410208 [3]中药成药性与制剂制备湖南省重点实验室,湖南长沙410208

出　　处：《中国中医药信息杂志》2018年第2期69-73,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81573691);湖南省重点实验室建设项目(2016TP1017);湖南省自然科学基金(2017JJ3236);湖南省教育厅科学研究项目(17B200)

摘　　要：目的采用超高液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF/MS)方法对桑叶的化学成分进行快速分析。方法采用ACQUITY UPLC-Q-TOF/MS联用仪,以乙腈-水(含0.1%甲酸)为流动相梯度洗脱,流速0.3 m L/min,电喷雾离子源负离子模式采集检测,以Masslynx4.1软件分析数据。结果根据质谱所提供的保留时间、精确相对分子质量及二级质谱裂解碎片信息并结合文献进行分析,共鉴定和推测了桑叶中19种化学成分。结论该方法可快速、灵敏、全面地分析桑叶的化学成分,为探讨桑叶的药效物质基础提供依据。Obejective To conduct fast identification analysis on chemical constituents of Mori Folium UPLC-Q-TOF/MS method. Methods ACQUITY UPLC-Q-TOF/MS spectrometer was used, acetonitrile and water （containing 0.1% formic acid） as mobile phase with gradient elution. The flow rate was 0.3 mL/min, with ESI negative ion mode detection, data analysis with Masslynx4.1 software. Results According to the retention time provided by the mass spectrometry, the exact molecular weight and the secondary mass spectrometry were used to analyze the fragments by referring to literature, and 19 chemical constituents of Mori Folium were identified and deduced. Conclusion The method can analyze the chemical constituents of Mori Folium rapidly, sensitively and comprehensively, and provide the basis for the study of the pharmacological basis of Mori Folium.

关 键 词：桑叶 超高液相色谱-四级杆-飞行时间质谱 化学成分 黄酮 

分 类 号：R284.1[医药卫生—中药学]