精卵融合相关基因CD9和CD81在绵羊组织中的表达分析  

Tissue Expression Analysis of CD9 and CD81 Genes Associated with Sperm-egg Fusion in Sheep(Ovis aries)

作　　者：汤继顺 狄冉[1] 刘秋月[1] 王翔宇[1] 张效生[3] 张金龙[3] 胡文萍[1] 储明星[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所/农业部动物遗传育种与繁殖重点实验室,北京100193 [2]安徽省农业科学院畜牧兽医研究所,合肥230031 [3]天津市畜牧兽医研究所,天津300381

出　　处：《农业生物技术学报》2018年第1期87-95,共9页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.31501941);国家肉羊产业技术体系专项(CARS-38);中央级公益性科研院所基本科研业务费专项(No.Y2017JC24;No.2017ywf-zd-13;No.2013ywf-zd-1;No.2015ywf-zd-2和No.2015ywf-zd-8);中国农业科学院科技创新工程(ASTIP-IAS13;CAAS-XTCX2016010-01-03;CAAS-XTCX2016010-03-03;CAAS-XTCX2016011-02-02);宁夏农林科学院科技创新先导资金项目(DWJLC-2016001);内蒙古自治区科技重大专项;农业科研杰出人才及其创新团队项目;国家万人计划科技创新领军人才项目

摘　　要：CD9和CD81是哺乳动物精卵融合重要的候选因子,其在机体内组织表达情况尚不清楚。为了初步探讨CD9和CD81基因在绵羊(Ovis aries)组织中的表达情况,本研究利用半定量反转录-聚合酶链式反应(semi-quantitative reverse transcription polymerase chain reaction,sqRT-PCR)和实时荧光定量PCR(quantitative real-time PCR,qRT-PCR)技术对两个基因在卵泡期小尾寒羊(高繁殖力)和苏尼特羊(低繁殖力)各组织中的表达进行了比较分析。结果表明,CD9和CD81基因在两种绵羊的14种组织中均有表达;CD9基因表达量在两个品种绵羊输卵管组织中都最高,且在小尾寒羊下丘脑、垂体和卵巢组织中的表达都极显著高于苏尼特羊(P<0.01);在小尾寒羊卵巢和下丘脑组织中的表达显著高于垂体和子宫(P<0.05),在苏尼特羊下丘脑和子宫组织中的表达显著高于卵巢和垂体(P<0.05)。CD81基因在小尾寒羊繁殖轴组织中的表达均极显著高于苏尼特羊(P<0.01);CD81基因表达量在小尾寒羊下丘脑组织中最高(P<0.01),在垂体和输卵管组织中的表达极显著高于卵巢和子宫(P<0.01);CD81基因表达量在苏尼特羊卵巢组织中最高(P<0.01),在下丘脑组织中的表达极显著高于垂体、输卵管和子宫(P<0.01)。研究结果提示CD9和CD81基因可能与绵羊繁殖力有关,为初步揭示绵羊繁殖性能的分子调控机制提供了参考。CD9 and CD81 were both important candidate factors during sperm-egg fusion in mammals, but their tissue expression characteristics in vivo were still unknown. In order to elucidate their expression characteristics in sheep （Ovis aries）, tissue expression of CD9 and CD81 genes in Small Tail Han sheep ewes （high fecundity） and Sunite sheep ewes （low fecundity） in the follicular phase was investigated using semi-quantitative reverse transcription polymerase chain reaction （sqRT-PCR） and quantitative real-time quantitative PCR （qRT-PCR） in the present study. The results showed that the expression of CD9 and CD81 genes was found in 14 tissues of two sheep breeds. The CD9 gene expression was the highest in oviduct tissue of two sheep breeds, and its expression in hypothalamus, pituitary and ovary tissues of Small Tail Han sheep was extremely higher than that of Sunite sheep （P〈0.01）. The CD9 gene expression in ovary and hypothalamus tissues was significantly higher than that in pituitary and uterus in Small Tail Han sheep （P〈0.05）, while its expression in hypothalamus and uterus tissues was significantly higher than that in ovary and pituitary in Sunite sheep （P〈0.05）. The expression of CD81 gene in reproductive axis tissues in Small Tail Han sheep was extremely higher than that in Sunite sheep （P〈0.01）. In Small Tail Han sheep, the CD81 gene expression was the highest in hypothalamus tissue （P〈0.01）, and its expression in pituitary and oviduct tissues was extremely higher than that in ovary and uterus tissues （P〈0.01）. While in Sunite sheep, the CD81 gene expression was the highest in ovary tissue （P〈0.01）, and its expression in hypothalamus was extremely higher than that in pituitary, oviduct and uterus （P〈0.01）. Our study preliminarily indicated that CD9 and CD81 genes maybe relate with difference of sheep prolificacy, which might provide a reference for revealing the molecular regulatory mechanism of sheep reproductive performance.

关 键 词：绵羊 精卵融合 CD9基因 CD81基因 组织表达 

分 类 号：S811[农业科学—畜牧学]