中华绒螯蟹蜕壳周期内蜕皮激素和蜕壳相关基因的表达动态分析  

Dynamic Analysis of Ecdysteroid Hormone Content and Molting Related Genes Expression in the Molting Cycle of Chinese Mitten Crab(Eriocheir Sinensis)

作　　者：黄姝[1] 陈娇[1] 陈晓雯[1] 陈义培 岳武成 王军[1] 王成辉[1] 

机构地区：[1]上海海洋大学农业部淡水水产种质资源重点实验室/水产科学国家级实验教学示范中心/上海水产养殖工程技术研究中心,上海201306

出　　处：《农业生物技术学报》2018年第1期150-158,共9页Journal of Agricultural Biotechnology

基　　金：上海市中华绒螯蟹产业技术体系项目(沪农科(产)字2017第4号);上海市科委项目(No.16391953000);上海市工程技术中心建设项目(No.13DZ2251800);上海市农委科技兴农重点攻关项目(No.G2017-02-08-00-10-F00076)

摘　　要：蜕壳是甲壳动物重要的生物学现象,中华绒螯蟹(Eriocheir sinensis)的生长发育和断肢再生都是通过蜕壳完成的。为了解中华绒螯蟹蜕壳生长的调控机制,本研究对不同蜕壳阶段的蜕皮激素(ecdysteroid hormone,EH)含量、蜕皮激素受体基因(ecdysone receptor,EcR)与蜕皮抑制激素基因(moltinhibiting hormone,MIH)的表达量进行了测定,并分析了其与生长性状的关联性。结果表明,EH含量在蜕壳周期内呈规律地波动性变化,当其含量达到约12 U/mL且持续上升时,中华绒螯蟹将启动蜕壳;EH含量与EcR的表达量呈极显著正相关(P<0.01),但与MIH的表达量呈极显著负相关(P<0.01);在蜕壳前期,EH的含量和EcR的表达量均显著高于蜕壳后期和间期(P<0.05),随着蜕壳的进行,EH含量和EcR表达量逐步降低,蜕壳后期显著低于蜕壳前期(P<0.05),到蜕壳间期最低,显著低于其他时期(P<0.05);而MIH在不同蜕壳阶段的表达模式正好与两者相反。通过与生长性状的关联性分析表明,蜕壳前期和间期的EH含量与体重呈显著正相关(P<0.05);不同蜕壳阶段的EcR表达量与体重均呈显著正相关(P<0.05),且蜕壳间期的表达量还与壳长、壳宽呈显著正相关(P<0.05);MIH在脱壳前期和脱壳间期的表达量与体重呈极显著负相关(P<0.01),且蜕壳间期的表达量与壳长、壳宽呈极显著负相关(P<0.01)。本结果为中华绒螯蟹蜕壳生长的分子机制及其蜕壳后增重差异的分子与生理基础提供了理论依据。Molting is a vital biological phenomenon of crustaceans and is closely related to the growth and leg regeneration of Chinese mitten crab （Eriocheir sinensis）. In order to detect the mechanism of molting and growth of Chinese mitten crab, the concentration of ecdysteroid hormone （EH）, the expression of ecdysone receptor gene （EcR） and molt-inhibiting hormone （MIH） gene at different molting stages were investigated by ELISA and qRT-PCR, respectively. Meanwhile, the correlation analysis was also conducted between growth traits and the concentration of EH, expression of EcR or MIH genes. The results showed that the concentration of EH fluctuated regularly in one molt cycle, and molting would started when EH content reached about 12 U/mL. The concentration of EH was significantly positive correlation with expression of EcR（P〈0.01）, but was significantly negative correlation with expression of MIH（P〈0.01）. The concentration of EH and expression of EcR at premolt stage were significant higher than that at postmolt stage （P〈0.05）, and they were the lowest at intermolt stage compared with that at other molting stages （P〈0.05）. However, the expression of MIH showed completely opposite trend compared with the concentration of EH and expression of EcR, indicating the highest at intermolt stage compared with that at other molting stages （P〈0.05）. There was a significantly negative correlation for the expression of EcR between intermolt and postmolt stage （P〈0.05）, and significantly positive correlation for the expression of MIH between premolt and intermolt stage, as well as between premolt and postmolt stage （P〈0.05）, while there was no significant correlation for the EcR and MIH genes at other molting stages （P〉0.05）, which indicated that the expression of MIH at premolt stage had a remarkably effect on the expression of MIH at postmolt and intermolt stage; while the expression of EcR at intermolt stage had a significant effect on the expression of EcR at postm

关 键 词：中华绒螯蟹 蜕壳阶段 蜕皮激素 基因表达 

分 类 号：S917.4[农业科学—水产科学]