猪支气管败血波氏杆菌生物被膜态和常规培养比较蛋白组学分析  

Comparative Proteomic Analysis Between Biofilm-forming Cells and Planktonic Cells of Swine Brodetella Bronchiseptica

作　　者：宁建刚[1] 高翔[1] 肖敏[1] 冯娜[1] 温峰琴[1] 包世俊[1] 邢小勇[1] 胡永浩[1] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070

出　　处：《农业生物技术学报》2018年第1期159-166,共8页Journal of Agricultural Biotechnology

基　　金：甘肃农业大学盛彤笙基金(GSAU-STS-1523);甘肃农业大学动物医学院教研产学创新基金(JYCX-KX008)

摘　　要：猪支气管败血波氏杆菌(Bordetella bronchiseptica,Bb)是引起猪萎缩性鼻炎(Swine infectious atrophic rhinitis,AR)的病原之一;本研究首先对支气管败血波氏杆菌生物被膜的形成条件进行优化,结果表明,培养时间为24 h、Na Cl浓度为0.4%、pH为7.5、乳糖浓度为0.5%是支气管败血波氏杆菌生物被膜形成的最适条件。进而利用比较蛋白质组学方法,研究了生物被膜态和常规培养下全菌蛋白的表达差异,明确差异蛋白的功能。通过双向电泳(two-dimensional gel electrophoresis,2-DE)比较了两种状态下全菌蛋白的表达差异,结果发现在生物被膜态的全菌蛋白中,有15个蛋白点表达上调,9个蛋白点表达下调。经质谱鉴定,上调的蛋白点主要有延伸因子(elongation factor Tu,EF-Tu)、超氧化物歧化酶(superoxide dismutase,SOD)、分子伴侣(molecular chaperone)等,功能主要体现在应激、调控、代谢方面。该结果从蛋白质水平上深化了对Bb生物被膜形成机制的理解,并为AR的防控和深入研究提供参考。Bordetella bronchiseptica （Bb） is one of the pathogens of Swine infectious atrophic rhinitis （AR）. Optimal conditions of Bb biofilm formation were developed, biofilm was well developed after 24 h, concentration of NaCl at 0.4%, pH 7.5, concentration of lactose at 0.5%. The protein expression profiles of whole Bb planktonic cells and biofilm-forming cells were investigated by comparative proteomics analysis, In this study, we compared the expression patterns of the whole Bb bacterial proteins originated from planktonic cells and biofilm-forming cells by a two-dimensional gel electrophoresis （2-DE）. The results revealed that 15 protein spots were up-regulated and nine were down-regulated. Identified by mass spectrometry, the up-regulated protein spots mainly included elongation factor Tu （EF-Tu）, superoxide dismutase （SOD） and molecular chaperone, involved in the stress, regulation and metabolism. The study is helpful to understand the Bb biofilm formation mechanism, and to prevent and control AR.

关 键 词：猪 支气管败血波氏杆菌 比较蛋白组学 生物被膜态 常规培养 

分 类 号：S855.12[农业科学—临床兽医学]