HPLC测定脑得生固体分散体胶囊中山楂黄酮类成分的含量  

Determination of Hawthorns Flavonoids in Naodesheng Solid Dispersion Capsules by HPLC

作　　者：罗兰 李明丽[1] 康家珍 王淑美 梁生旺 

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]广东药科大学国家中医药管理局中药数字化质量评价技术重点研究室,广东广州510006 [3]广东药科大学广东高校中药质量工程技术研究中心,广东广州510006

出　　处：《中华中医药学刊》2018年第1期64-66,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81274059)

摘　　要：目的：建立脑得生固体分散体胶囊中山楂黄酮类成分（牡荆素鼠李糖苷、牡荆素、金丝桃苷）的含量测定方法。方法：色谱柱Hyspersil ODS-2（5μm,250 mm×4.6 mm）,柱温25℃,流动相乙腈-甲醇-四氢呋喃-0.04%磷酸水（1∶1∶19.4∶78.6）,流速0.9 mL·min-1,检测波长370 nm。结果：牡荆素鼠李糖苷在0.167 21.003 2μg（r=0.999 9）范围,牡荆素在0.124 80.748 8μg（r=0.999 9）范围,金丝桃苷在0.276 01.656 0μg（r=0.999 9）范围均呈良好的线性关系;平均回收率分别为97.27%,99.12%,99.09%;RSD分别为1.57%,2.15%,2.71%。结论：所建立方法简便、准确、重复性好,可用于控制该制剂的质量。Objective： To simultaneously determine the contents of vitexin-2-O-rhamnoside,vitexin and hyperoside in Naodesheng Solid Dispersion Capsules by HPLC. Methods： An external standard method by HPLC with Hyspersil ODS-2 C18 column（5 μm,250 mm × 4. 6 mm） was used with the column temperature as 25 ℃. The detection wavelength was 370 nm. The flow rate was set at 0. 9 mL·min-1 and the mobile phase was acetonitrile-ethanol-tetrahydrofuran-0. 04% phosphoric acid solution（1 ∶ 1 ∶ 19. 4 ∶ 78. 6）. Results： Linearities of vitexin-2-O-rhamnoside,vitexin and hyperoside were good in the ranges of 0. 167 2 1. 003 2 μg（ r = 0. 999 9）,0. 124 8 0. 748 8 μg（ r = 0. 999 9）,0. 276 0 1. 656 0 μg（ r = 0. 999 9）,respectively. The average recoveries of vitexin-2-O-rhamnoside,vitexin and hyperoside were 97. 27%,99. 12%,99. 09% and RSD values were 1. 57%,2. 15%,2. 71%,respectively. Conclusion： The method is simple,accurate and reproducible,and can be used for quality control of Naodesheng Solid Dispersion Capsules.

关 键 词：脑得生固体分散体胶囊 HPLC 牡荆素鼠李糖苷 牡荆素 金丝桃苷 

分 类 号：R284.1[医药卫生—中药学]