益气解毒活络中药组分配伍对高糖高脂环境下人肾小球细系膜细胞NF-κB蛋白表达影响  

Effects of Compatibility of Chinese Herbal Medicine on Expression of NF-κB in Human Glomerular Mesangial Cells under High-glucose and High-fat Environment

作　　者：辛彩虹[1] 李峥[2] 李敬林[2] 旷劲松[1] 

机构地区：[1]沈阳市第四人民医院,辽宁沈阳110031 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《中华中医药学刊》2018年第1期83-86,I0008,I0009,共6页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81173153)

摘　　要：目的：观察益气解毒活络中药组分配伍对高糖高脂环境下人肾小球细系膜细胞NF-κB蛋白表达影响,探究益气解毒活络中药有效成分防治糖尿病肾病的机制。方法：体外培养人肾小球系膜细胞株,分为正常对照组,高糖高脂模型组、中药组（第1组、第2组、第3组）;Western Blot法检测0、15、30、60、90 min高糖高脂模型组NF-κB蛋白表达;Western Blot法检测60、90 min 5组NF-κB蛋白表达;免疫荧光染色检测NF-κB核转运。结果：（1）模型组第90 min NF-κB蛋白表达达到峰值。（2）各中药配伍组中第3组下调60 min NF-κB蛋白表达效果最佳;第1组下调90 min NF-κB蛋白表达效果最佳。（3）各组60、90 min NF-κB核转运比较发现：细胞免疫荧光显示,正常对照组p65主要分布在细胞质部分,模型对照组p65主要分布在细胞核部分,各中药配伍组p65明显分布在细胞质内,提示各中药配伍组60、90 min的NF-κB核转运作用被抑制。结论：益气解毒活络中药组分配伍可能是通过抑制NF-κB信号途径来保护受损的肾小球系膜细胞,从而起到减缓DN病程进展,保护受损肾脏的作用。Objective： To observe the effect of Yiqi Jiedu Huoluo Chinese medicine on NF-κB protein expression in human glomerular mesangial cells under high glucose and high fat environment and explore the mechanism of active ingredients of Yiqi Jiedu Huoluo Chinese medicine in preventing and treating diabetic nephropathy. Methods： Human glomerular mesangial cells were cultured in vitro and divided into normal control group,high glucose and high fat model group,traditional Chinese medicine group（ first group,second group,third group）. Western blot analyzed expression of NF-κB in the high glucose and high fat model group at 0,15,30,60 and 90 min. Western blotting detected five groups’ NF-κB protein expressions at 60 and 90 min. The immunofluorescence staining was used to detected the NF-κB nuclear transportation. Results：The expression of NF-κB protein in the model group at 90 min reached the peak value. The expression of NF-κB in the third group was better than that in the third group,and the expression of NF-κB was the best in the first group. The expression of p65 in the normal control group was mainly distributed in the cytoplasmic part,and the p65 of the model control group was mainly distributed in the nucleus. The p65 of the Chinese herbal medicine group was mainly distributed in the cytoplasm. The expression of p65 in the control group was significantly higher than that in the control group,suggesting that the NF-κB nuclear translocation was inhibited at 60 min and 90 min in the compatibility group. Conclusion： The combination of active and detoxifying traditional Chinese medicine components may be through the inhibition of NF-κB signaling pathway to protect damaged glomerular mesangial cells,which plays a role in slowing the progression of DN and protecting the damaged kidney.

关 键 词：益气解毒活络中药 糖尿病肾病 肾小球系膜细胞 NF-ΚB 

分 类 号：R285.5[医药卫生—中药学]