艾灸与针刺治疗炎症性肠病大鼠内脏痛的镇痛效应研究  

Research on Effects of Moxibustion and Acupuncture for Treating Visceral Pain of Rats Model with Inflammatory Bowel Disease

作　　者：张丹[1,2] 李志元[1] 黄燕 杨延婷[1] 施征[2] 吴焕淦[1,2] 马晓芃[1,2] 

机构地区：[1]上海中医药大学,上海201206 [2]上海市针灸经络研究所,上海200030 [3]上海市黄浦区中西医结合医院,上海200010

出　　处：《中华中医药学刊》2018年第1期94-99,I0011,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81202754,81273843);国家重点基础研究发展计划(973计划)项目(2015CB554501);上海市卫生与计划生育委员会项目(20144Y0153)

摘　　要：目的：观察艾灸、针刺（天枢和气海穴）对炎症性肠病大鼠内脏痛的影响,从痛行为、痛情绪及血清P物质含量角度对比观察艾灸与针刺治疗炎症性肠病内脏痛的效应异同。方法：SD（Sprague Dawley）大鼠随机分为正常组、模型组、艾灸组及针刺组,采用三硝基苯磺酸合50%乙醇灌肠的方法制备炎症性肠病内脏痛模型。通过检测大鼠腹壁收缩反射阈值、机械性缩足反射阈值、热缩足潜伏期观察大鼠痛行为变化,应用旷场实验、高架十字迷宫测验观察大鼠痛情绪变化,运用苏木素-伊红染色法观察结肠组织形态学变化,采用酶联免疫吸附测定法检测血清P物质含量。结果：与正常组比较,炎症性肠病模型组大鼠结肠损伤严重,表现为溃疡、炎症反应,经艾灸、针刺治疗后大鼠结肠损伤均有所减轻。与正常组比较,模型组大鼠不同压力刺激下腹壁收缩反射评分显著升高,机械性缩足反射时间、热缩足潜伏期缩短（均P〈0.01）,呈现内脏高敏和痛阈降低。与模型组比较,艾灸组、针刺组大鼠治疗后不同压力刺激下腹壁收缩反射评分明显降低,机械性缩足反射时间、热缩足潜伏期延长（均P〈0.01）。与针刺组比较,艾灸组大鼠治疗后在40、60 mmHg压力刺激下腹壁收缩反射评分降低明显（P〈0.01,P〈0.05）。与正常组比较,模型组大鼠活动总路程、直立次数、开臂时间及开臂次数均明显减少（均P〈0.01）,经艾灸、针刺治疗后大鼠活动总路程、直立次数、开放臂时间及次数比例增加（均P〈0.01）。与艾灸组比较,针刺组大鼠治疗后直立次数升高明显（P〈0.05）。与正常组比较,模型组大鼠血清P物质含量升高,经艾灸、针刺治疗后血清P物质含量降低（均P〈0.01）。与针刺组比较,艾灸组大鼠血清P物质含量下降明显（P〈0.01）。结论：艾灸、针刺（天枢和气海穴）对炎症性肠病内脏痛大�Objective：To observe the influence of moxibustion and acupuncture on Tianshu（ ST25）,Qihai（ CV6） on visceral pain of rats with inflammatory bowel disease and compare the similarities and differences of their effects from the point of pain-related behavior and emotion and content of P substance in serum. Methods： SD rats were randomly divided into four groups as a normal group,a model group,a moxibustion group and an acupuncture group. Rat model with inflammatory bowel disease and represented visceral pain was established by enema with trinitrobenzene sulfonic acid and 50% ethyl alcohol. Abdominal withdrawal reflex（ AWR） score,mechanical withdrawal threshold（ MWT） and thermal withdrawal latency（ TWL） were surveyed to inspect visceral sensitivity and pain related behavior. The Open Field Test and Elevated Plus Maze Test were executed for estimating pain related emotion. Hematoxylin-eosin staining was used to observe changes in colonic histomorphology. Enzyme-linked immunosorbent assay was adopted to detect content of P substance in serum. Results： Compared with normal group,the colonic damage of rats model with inflammatory bowel dis-ease was severe,manifested with ulcers and inflammation and injury of colon was alleviated to some extent after moxibustion and acupuncture treatment. Compared to normal group,AWR scores of rats in model group under different expansion pressure stimulus were significantly increased and their MWT and TWL were shortened（ all P 〈 0. 05）,indicating visceral hypersensitivity and low threshold of pain. Compared with model group,AWR scores of rats under different expansion pressure stimulus were reduced in moxibustion group and acupuncture group and their MWT and TWL were also prolonged after treatment（ all P 〈 0. 05）. Compared with acupuncture group,AWR scores in moxibustion group were markedly decreased at 40 mmHg and 60 mmHg expansion pressure stimulus（ P 〈 0. 01,P 〈 0. 05）. Compared to normal group,the total distance of horizontal motion,number

关 键 词：炎症性肠病 内脏痛 针刺 艾灸 异同 

分 类 号：R245.81[医药卫生—针灸推拿学] R245.31[医药卫生—中医临床基础]