艾可清对艾滋病猴T细胞各亚群的影响  

Effects of Aikeqing on T Lymphocyte Subsets of SIV/SAIDS

作　　者：熊思艺 陈剑涛[1] 张远芬[1] 符林春[1] 

机构地区：[1]广州中医药大学热带医学研究所

出　　处：《中华中医药学刊》2018年第1期173-176,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81173436,81302897);国家科技重大专项项目(2014ZX10005002)

摘　　要：目的：探讨艾可清对艾滋病猴T细胞各亚群的免疫调节作用。方法：选取经静脉注射感染SIVmac239的中国恒河猴15只,随机分为3组：艾可清低剂量组,艾可清高剂量组和模型对照组,每组5只。分别于给药前、给药4周、给药8周、停药4周和停药8周采集外周血,检测T细胞功能亚群、纯真亚群和记忆亚群,观察其变化;分离血浆检测外周血SIV病毒载量。结果：与对照组相比,高剂量组CD4＋CD28-（%）在给药第4周、第8周和停药4周均显著上升（P〈0.05,P〈0.01）;CD4＋纯真细胞在高剂量组给药8周显著上升（P〈0.05）,CD8＋纯真细胞在低剂量组给药第4周、第8周和高剂量组给药8周均显著上升（P〈0.05,P〈0.01）;高、低剂量组各时间点病毒载量与对照相比没有显著变化。结论：艾可清对艾滋病猴CD4＋CD28-细胞和CD4＋、CD8＋纯真细胞有一定的提升作用。Objective： To study the immune regulation effect of Aikeqing on T lymphocyte subsets of SIV/SAIDS.Methods： Fifteen Chinese rhesus monkeys infected with SIVmac239 were randomly divided into 3 groups： low dose Aikeqing group,high dose Aikeqing group and model control group,5 rats in each group. Then the functional subsets,naive subsets and memory subsets of T cell were detected and peripheral blood viral load was tested in pre-administration,4 and 8 weeks during administration and 4 and 8 weeks after drug withdrawal. Results： Compared with the control group,the percentage of CD4＋ CD28 cells in high does group raised significantly at the 4 th and 8 th week in administration and 4 th week after drug withdrawal（ P 〈 0. 05,P 〈 0. 01）. CD4＋ naive cells in high dose group were raised significantly at the 8 th week during administration（ P 〈 0. 05） and CD8＋ naive cells were raised significantly at the 4 th and 8 th week in the low dose group and the 8 th week in the high dose group（ P 〈 0. 05,P 〈 0. 01）. There was no significant change in viral load between the high and low dose groups at all time points compared to the control. Conclusion：Aikeqing can enhance CD4＋ CD28- cells and CD4＋ and CD8＋ naive cells in SIV/SADIS.

关 键 词：猴艾滋病 艾可清 功能细胞 记忆细胞 纯真细胞 

分 类 号：R285.5[医药卫生—中药学]