海洋沉积物来源草酸青霉T4-6化学成分及其生物活性研究  

Chemical Constituents of Marine Sediment-Derived Fungus Penicillium oxalicum T4-6 and Their Biological Activities

作　　者：马新玥 方圣涛[2] 赵业 季乃云[2] 

机构地区：[1]烟台大学,山东烟台264005 [2]中国科学院烟台海岸带研究所,山东烟台264003

出　　处：《化学与生物工程》2018年第1期17-22,共6页Chemistry & Bioengineering

基　　金：中国科学院战略性先导科技专项(A类)(XDA11020702)

摘　　要：从采集自渤海沉积物样品中分离得到一株海洋真菌草酸青霉T4-6,对其前期发酵条件进行调控和扩大发酵,所得发酵产物利用硅胶柱层析、Sephadex LH-20凝胶柱层析、反相硅胶柱层析、制备薄层层析、制备高效液相色谱等分离手段得到11个单体化合物,通过~1HNMR、^(13)CNMR等鉴定其分别为secalonic acid D(Ⅰ)、ω-hydroxyemodin(Ⅱ)、isorhodoptilometrin(Ⅲ)、oxalicine B(Ⅳ)、decaturin B(Ⅴ)、decaturin F(Ⅵ)、penipanoid C(Ⅶ)、cyathisterone(Ⅷ)、herbarulide(Ⅸ)、dankasterones A(Ⅹ)、volemolide(Ⅺ)。其中,化合物Ⅵ对赤潮异弯藻和海洋卡盾藻的生长具有较强的抑制作用,化合物Ⅰ、Ⅱ对海洋卡盾藻的生长具有较好的抑制作用,化合物Ⅰ对枯草杆菌和炭疽杆菌具有良好的抑制作用。We separated a strain of marine fungus Penicilliurn oxalicum T4-6 from the sediments of Bohai Sea. Through preliminary optimization of fermentation conditions, we obtained eleven compounds by silica gel column chromatography,Sephadex LH-20 gel column chromatography, reversed phased silica gel column chromatography,preparative thin layer chromatography, and preparative high performance liquid chromatography. Moreover,we identified their structures by ^1HNMR and ^13CNMR as secalonic acid D（ Ⅰ ）,ω-hydroxyemodin （ Ⅱ ） ,isorhodoptilometrin（ Ⅲ ）, oxalicine B（ Ⅳ ）, decaturin B（ Ⅴ ）, decaturin F（ Ⅵ ）, penipanoid C（ Ⅶ ）, cyathisterone（ Ⅷ ）, herbarulide（ Ⅸ ）, dankasterones A（ Ⅹ ）, and volemolide（ Ⅺ ）. Among them, compound Ⅵ shows obvious anti-microalgae activities against Heterosigma akashiwo and Chattonella marina. Additionally, compounds Ⅰ and Ⅱ show good anti-microalgae activities against Chattonella marina ,and compound Ⅰ possesses antibacterial activities against Bacillus subtilis and Bacillus anthracis.

关 键 词：草酸青霉 化学成分 次级代谢产物 抗微藻活性 抗菌活性 

分 类 号：Q949.32[生物学—植物学]