双黄连对MHV-3诱导的暴发型肝衰竭小鼠的保护作用研究  

Protective effects of Shuanghuanglian,a herbal compound,in mice with MHV-3-induced fulminant liver failure

作　　者：黄明[1] 邓卫平[2] 彭敏[2] 吴胜英[3] 

机构地区：[1]湖北省十堰市中医医院,442012 [2]十堰太和医院 [3]湖北医药学院

出　　处：《实用肝脏病杂志》2018年第1期46-49,共4页Journal of Practical Hepatology

基　　金：湖北省教育厅指导项目(编号:B2016125)

摘　　要：目的探讨双黄连对病毒感染诱导的暴发性肝衰竭小鼠的保护作用。方法取BABL/c J小鼠36只,随机均分为对照组、模型组和双黄连处理组。取滴度为1×106 PFU/ml的3型鼠肝炎病毒(MHV-3),采用高压水注射法经小鼠尾静脉注入小鼠体内,诱导暴发性肝衰竭模型。在造模后给予10%双黄连或生理盐水灌胃治疗7 d。采用RT-PCR法检测小鼠肝组织IP-10 mRNA和血清MHV-3 DNA水平,并检测血清转化生长因子-βl(TGF-βl)、白细胞介素-10(IL-10)、层黏连蛋白(LN)、透明质酸(HA)和肝功能指标。结果在治疗7 d,模型组和双黄连组小鼠血清谷丙转氨酶(ALT)分别为(229.03±30.56)IU/L和(22.83±6.02)IU/L,谷草转氨酶(AST)为(139.01±11.75)IU/L和(32.45±4.59)IU/L,LN分别为(99.86±10.57)nmol/L和(57.02±7.24)nmol/L,HA为117.05±16.72)nmol/L和(53.01±11.35)nmol/L,TGF-βl分别为(319.46±39.12)pg/ml和(93.87±11.20)pg/ml,IL-10为25.70±3.87)pg/ml和(1.07±0.09)pg/ml,MHV-3 DNA分别为12.47±3.05)lg copies/mL和(1.24±0.10)lg copies/mL,肝组织IP-10 mRNA分别为【(25.70±3.87)和(12.79±3.08),P<0.05】。结论双黄连有一定的抗MHV-3作用,可能是通过抑制肝内IP-10 mRNA水平,减少IL-10对肝细胞的炎性损伤,阻止了肝组织纤维化而促进了肝功能的恢复。Objective To investigate the protective effects of Shuanghuanglian,a herbal compound,in mice with murine hepatitis virus strain 3 （MHV-3）-indueed fulminant liver failure （FLF）. Methods 36 BABL/36 eJ mice were randomly divided into control,model and Shuanghuanglian-intervened group,and the model of FLF was established by intravenous injection of 1 x10^6 PFU/ml MHV-3. 10% of Shuanghuanglian compound or normal saline was given by garage for 7 days after the model was successfully established. The ehemokine IP-IO mRNA in liver tissues and serum MHV-3 DNA loads were quantitatively assayed by RT-PCR,and serum transforming growth factor beta l（TGF- beta 1）,intedeukin-10 （IL-10）,laminin（LN）,hyaluronic acid （HA） and liver function index were detected routinely. Results Serum ALT levels in the model and Shuanghuanglian-intervened group at end of 7 days were （229.03±30.56） U/L and （22.83±6.02） IU/L,AST were （139.01±11.75） U/L and （32.45±4.59） U/L. LN were （99.86±10.57） nmol/L and （57.02±7.24） nmol/L, HA were （117.05±16.72） nmol/L and （53.01± 11.35）nmol/L,TGF-beta 1 were （319.46±39.12） pg/ml and （93.87±11.20） pg/ml,IL-10 were （25.70±3.87） pg/ml and （1.07±0.09） pg/ml,MHV-3 DNA were （12.47±3.05） lg copies/mL and （1.24±0.10） lg copies/mL,and IP-10 mRNA in liver tissues were [（25.70 ±3.87）and （ 12.79 ±3.08）, P〈0.051. Conclusion Shuanghuanglian, a herbal compound,has a certain effect of anti MHV-3 virus,and the mechanism by which it works might be through inhibiting the expression of IP-10 mRNA,redueing the inflammatory damage of IL-10 to the hepatoeytes, preventing liver fibrosis and promoting the recovery of liver functions.

关 键 词：肝衰竭 3型鼠肝炎病毒 双黄连 趋化因子IP-10 白细胞介素-10 小鼠 

分 类 号：R285.5[医药卫生—中药学]