马骨关节炎模型关节液中CTX-Ⅱ含量变化的研究  

Changes insynovial fluid CTX-Ⅱ from equine with experimentally-induced osteoarthritis

作　　者：马天文 王冠颖[1] 李玥[1] 李欣然[1] 姜仁礼 宋霄鹏 张志恒 白荟 李新[1] 陈宇航 高利[1] 

机构地区：[1]东北农业大学动物医学学院黑龙江省实验动物与比较医学重点实验室,黑龙江哈尔滨150030 [2]通辽市鲁北镇兽医站,内蒙古通辽029100

出　　处：《中国兽医杂志》2017年第11期13-15,19,共4页Chinese Journal of Veterinary Medicine

基　　金：十三五国家重点研发计划(2017YFD050220)

摘　　要：目前马骨关节炎(OA)的临床诊断尚缺少有效的生物标志物(BM)。为探讨马关节液Ⅱ型胶原羧基端端肽(CTX-Ⅱ)的浓度与OA发病程度的关系。试验选用13匹蒙古马,随机分为造模组(n=8)和对照组(n=5),在马左侧腕关节内注入2 mL两性霉素B(25 mg/mL)诱导OA,对照组马匹注入等量PBS作为对照,于造模后每周采集一次关节液,直至第9周。ELISA检测马关节液中的CTX-Ⅱ浓度。结果显示,与对照组相比,造模组从第1周开始,关节液中CTX-Ⅱ的浓度增加,从第2周开始显著增加(P<0.05),第4周开始极显著增加(P<0.01)并且保持增加的趋势到第9周。造模组马匹第3周开始出现不同程度跛行。试验结果表明,CTX-Ⅱ浓度的升高在跛行前就已经显著升高,推测CTX-Ⅱ可以作为马关节炎BM,为以后建立马OA的快速临床诊断技术提供理论依据。There are no effective biomarkers （BM） for the diagnosis of equine osteoarthritis currently. To investigate the rela- tionships between the concentration of C-terminal telopeptide of type Ⅱ collagen （ CTX -Ⅱ ） and the degree of OA in synovial fluid, a total of 13 Mongolia horses were randomly divided into model group （n = 8 ） which was injected with 2ml amphotericin B （25 mg/ ml） into the left wrist to induce osteoarthritis and control group （ n = 5 ） which was administrated with the same dose of PBS. Then, the synovial fluid was collected once a week after modeling until the 9th week to detect the concentration of CTX - Ⅱ by ELISA. The results indicated that the concentration of CTX - Ⅱ in the model group changed in a growing trend within 9 weeks compared to the control group, which rose sharply from the second week （ P 〈0. 05） and increased remarkably at the 4th week （ P 〈0. 01 ）. The horses in the model group appeared limping symptom in vary degres in the third week. The conclusion is that the growing concentration of CTX - Ⅱ has been significantly increased before lameness so that CTX - Ⅱ could be used as a biomarker of equine OA for the establishment of rapid clinical diagnosis of OA.

关 键 词：骨关节炎 两性霉素B CTX-Ⅱ 马 

分 类 号：S857.165[农业科学—临床兽医学]