腹泻患者艰难梭菌及其毒素A/B检出率分析  

Detection of Clostridiumdifficileand its toxins A/B in patients with diarrhea

作　　者：常洪美[1] 李炼[1] 凌冬[1] 张燕华[1] 陈玲[1] 陈建美[1] 

机构地区：[1]崇州市人民医院,四川崇州611230

出　　处：《中国微生态学杂志》2017年第12期1413-1415,共3页Chinese Journal of Microecology

基　　金：四川省预防医学会基金(2015SCGK003)

摘　　要：目的了解住院腹泻患者艰难梭菌及其毒素A/B的检出情况,为临床诊断抗生素相关性腹泻提供参考依据。方法收集2015年9月至2016年8月崇州市人民医院疑似抗生素相关性腹泻住院患者的粪便标本163份,用艰难梭菌快速检测试剂盒检测艰难梭菌特异性抗原谷氨酸脱氢酶(GDH);用酶联免疫荧光法检测GDH阳性标本中艰难梭菌毒素A/B的产生情况。结果 163份粪便标本中GDH阳性为34份,阳性率为20.86%。34份GDH阳性标本中艰难梭菌毒素A/B阳性率为41.18%(14/34),可疑阳性率为17.65%(6/34),阴性率为41.18%(14/34)。结论疑似抗生素相关性腹泻住院患者艰难梭菌GDH检出率较高,毒素A/B阳性率也比较高,提示临床应重视抗生素相关性腹泻患者艰难梭菌感染的诊断。Objective To understand the prevalence of Clostridium difficile and toxins A/B in hospitalized diarrhea patients and provide reference for the clinical diagnosis of antibiotic related diarrhea.Methods A total of 163 fecal samples were collected from antibiotic related diarrhea patients in our hospital from September 2015 to August 2016.Glutamate dehydrogenase（GDH）was detected by using Clostridium difficile detection kit.The toxins A/B in GDH positive specimens was detected by using enzyme-linked immunofluorescence method.Results 34 GDH positive specimens were found;the positive rate was 20.86%.The positive rate of toxins A/B in GDH positive specimens was 41.18%（14/34）;the suspicious rate was 17.65%（6/34）and the negative rate was 41.18%（14/34）.Conclusion The positive rates of GDH and toxins A/B were high in patients with antibiotic related diarrhea,suggesting that it is important to pay attention to the diagnosis of Clostridium difficile infection in antibiotic related diarrhea patients in clinical practice.

关 键 词：腹泻 艰难梭菌毒素A/B 特异性抗原 谷氨酸脱氢酶 酶联免疫荧光分析 

分 类 号：R446.1[医药卫生—诊断学]