利用InDel分子标记分析海南山栏稻品种籼粳特性  

Indica-japonica Differentiation of Shanlan Upland Rice Varieties from Hainan Revealed by InDel Markers

作　　者：徐建欣[1] 杨洁[1] 李美萱 敖莉丝 徐志军 

机构地区：[1]中国热带农业科学院湛江实验站/热带旱作农业研究中心,广东湛江524013 [2]岭南师范学院,广东湛江524048 [3]云南农业大学/农业生物多样性与植物病害控制教育部重点实验室,昆明650201

出　　处：《中国农学通报》2017年第36期7-13,共7页Chinese Agricultural Science Bulletin

基　　金：海南省自然科学基金"海南陆稻籼粳分化与抗旱性鉴定综合评价"(20153146);国家自然科学基金青年科学基金项目"中国热区抗旱优质陆稻种质资源筛选与遗传多样性研究"(31400280);中央级公益性科研院所基本科研业务费专项资金(院本级)"热带粮食作物种质资源收集;保存和创新利用团队(骨干岗位)"(17CXTD-03)

摘　　要：为明确海南山栏稻品种的籼粳分化特性与遗传变异规律,利用34个籼粳特异InDel分子标记和24个SSR分子标记对22个海南山栏稻品种的籼粳特性与遗传变异进行分析。InDel分子标记鉴定结果表明,供试品种粳型基因频率变幅在0.68~0.83,以粳型品种为主,占到总品种的63.6%,未发现籼型或偏籼型品种。海拔梯度分析表明,海南山栏稻中粳型品种分布范围更为广泛,具有更强的环境适应能力。遗传变异分析表明,在供试群体中共检测到191个等位基因,其中稀有等位基因(基因频率≤0.05)数为86个,占到总等位基因数的45.03%。在供试的3个地理来源中,琼中县的遗传多样性最为丰富。The aims are to clarify the indica-japonica differentiation and the genetic variation of Shanlan upland rice varieties from Hainan. In this study, 34 indica-japonica special In Del markers and 24 SSR molecular markers were used in 22 Shanlan upland rice varieties from Hainan to analyze the characteristics of indica-japonica differentiation and genetic variation. Results of InDel molecular markers showed that, gene frequency variation of japonica types ranged from 0.68 to 0.83. The japonica-type varieties were the main type,which accounted for 63.6%, while indica and partial indica were not found. The analysis of altitude gradient showed that, the distribution of japonica varieties of Shanlan upland rice in Hainan was more extensive and more adaptable. Genetic variation analysis showed that, 191 alleles were detected in the test varieties,including 86 rare alleles（gene frequency≤0.05）, which accounted for 45.03% of the total alleles. The richest genetic diversity of upland rice was found in Qiongzhong County of the three testing areas.

关 键 词：山栏稻 SSR分子标记 InDel分子标记 籼粳分化 遗传变异 

分 类 号：S511.6[农业科学—作物学]