新型鸭呼肠孤病毒病浓缩灭活疫苗的制备及免疫原性分析  

Efficacy Evaluation of a Concentrated Inactivated Vaccine against Novel Duckling Reovirus Infection

作　　者：陈仕龙[1] 肖世峰 程晓霞[1] 林锋强[1] 王劭[1] 王锦祥[1] 朱小丽[1] 陈少莺[1] 

机构地区：[1]福建省农业科学院畜牧兽医研究所/福建省畜禽疫病防治工程技术研究中心,福建福州350013

出　　处：《福建农业学报》2017年第11期1189-1192,共4页Fujian Journal of Agricultural Sciences

基　　金：福建省科技计划项目--省属公益类科研院所基本科研专项(2014R1023-9);福建省农业科学院青年英才项目(YC2016-1)

摘　　要：为研制高效的新型鸭呼肠孤病毒病(NDRV)灭活疫苗,分别采用病毒尿囊液和浓缩后病毒尿囊液为抗原,经甲醛灭活处理后与常规油佐剂制成灭活疫苗,并进行种鸭免疫后血清学检测、雏鸭免疫保护及安全试验。结果表明,浓缩疫苗安全性好,能明显提高疫苗的免疫效果,高峰期抗体效价的免疫持续期比常规疫苗长;浓缩疫苗免疫雏鸭后的免疫保护性达100%。以上研究表明,NDRV浓缩疫苗安全、高效,在雏鸭上具有良好的免疫保护效果,具有良好的市场前景。In this paper, an experimental concentrated inactivated vaccine and a conventional reactivated vaccine against Novel Duckling Reovirus （NDRV） infection were prepared. After one single-dose immunization in adult Cherry Valley ducks, the serum samples collected in a period of 13 weeks were tested for antibodies by serum neutralisation （SN） method. The protective effects against NDRV infection were observed in 3-day-old Cherry Valley ducklings and mule duckling after a single-dose immunization. The serological results showed that concentrated inactivated vaccine was much more immunogenic than the conventional inactivated vaccine, with a much higher SN antibody titers at all test time points. The concentrated inactivated vaccine was highly protective against NDRV challenge at 7-day post-vaccination with a 100% protective efficacy, as compared to 40%- 60% protective efficacy for conventional inactivated vaccine. Taken together, this experimental concentrated inactivated vaccine exhibited immune efficiency much superior to conventional inactivated vaccine and might be a useful vaccine against NDRV infection.

关 键 词：新型鸭呼肠孤病毒感染 浓缩疫苗 常规疫苗 免疫原性 

分 类 号：S858[农业科学—临床兽医学]