SRWD1, a novel target gene of DELLA and WRKY proteins, participates in the development and immune response of rice (Oryza sativa L.)  

作　　者：Chuanmiao Xie Ge Zhang Yuman Zhang Xiaoguang Song Hongyan Guo Xiaoying Chen Rongxiang Fang 

机构地区：[1]State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China [2]National Center for Plant Gene Research (Beijing), Beijing 100101, China [3]University of Chinese Academy of Sciences, Beijing 100049, China [4]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101. China

出　　处：《Science Bulletin》2017年第24期1639-1648,共10页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China (91217304);the National Transgenic Science and Technology Program (2016ZX08010-002)

摘　　要：SRWD1, a member of the WD40 protein subfamily, is induced by salt stress in rice and its homolog in barley can bind to GAMYB, implying that SRWD1 might be involved in plant defense against environmental stress and gibberellic acid(GA) signalings. In this study, we focused on the biological functions and regulation mechanisms of SRWD1 in rice. The results showed that SRWD1 expression was repressed by GA and induced by abscisic acid(ABA). Two WRKY-family transcription factors, Os WRKY45 and Os WRKY72, were found to regulate SRWD1 expression by directly binding to the W-box region in its promoter. Transient co-expression and yeast two-hybrid analyses showed that a DELLA protein strengthened the activation of Os WRKY45 and partly relieved the suppression of Os WRKY72 by binding to them.Interestingly, both SRWD1-overexpressing transgenic plants and SRWD1-knockout mutants showed dwarf phenotypes and resistance to Xanthomonas oryzae.SRWD1, a member of the WD40 protein subfamily, is induced by salt stress in rice and its homolog in barley can bind to GAMYB, implying that SRWDI might be involved in plant defense against environmental stress and gibberellic acid （GA） signalings. In this study, we focused on the biological functions and regulation mechanisms of SRWD1 in rice. The results showed that SRWD1 expression was repressed by GA and induced by abscisic acid （ABA）. Two WRKY-family transcription factors, OsWRKY45 and OsWRKY72, were found to regulate SRWD1 expression by directly binding to the W-box region in its promoter. Transient co-expression and yeast two-hybrid analyses showed that a DELLA protein strengthened the activation of OsWRKY45 and partly relieved the suppression of OsWRKY72 by binding to them. Interestingly, both SRWD1-overexpressing transgenic plants and SRWD1-knockout mutants showed dwarf phenotypes and resistance to Xanthomonas oryzae.

关 键 词：PHYTOHORMONE Gibberellin(GA) Abscisic acid(ABA) Signal transduction DELLA protein WRKY family protein 

分 类 号：S511[农业科学—作物学]