血吸虫对吡喹酮抗药性的研究ⅩⅥ 日本血吸虫吡喹酮抗性株在钉螺体内的生物学特性  

Studies on resistance of Schistosoma to praziquantel ⅩⅥ Biological characteristics of praziquantel-resistant isolates of Schistomoma japonicum in Oncomelania hupensis snails

作　　者：钱科[1,2] 梁幼生[1] 汪伟[1] 曲国立[1] 李洪军[1] 杨振坤[1] 赵正阳[1] 邢云天[1] 戴建荣[1] 

机构地区：[1]国家卫生和计划生育委员会寄生虫病预防与控制技术重点实验室,江苏省寄生虫与媒介控制技术重点实验室,江苏省血吸虫病防治研究所,江南大学公共卫生研究中心,无锡214064 [2]江西省南昌市疾病预防控制中心

出　　处：《中国血吸虫病防治杂志》2017年第6期678-682,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家科技支撑计划(2009BAI78B06);国家自然科学基金(30471516);江苏省自然科学基金(BK2004024;BK20141105);江苏省科教强卫工程项目;江苏省青年医学人才项目(QNRC2016621);江苏省无锡市卫生局重大科研项目(Z201507)

摘　　要：目的观察、比较日本血吸虫吡喹酮抗性株和敏感株在中间宿主钉螺体内无性生殖阶段的生物学特性差异。方法采用实验室诱导获得的日本血吸虫吡喹酮抗性株和保种传代的敏感株尾蚴定量感染小鼠,感染37 d后分别收集敏感株和抗性株虫卵,孵化毛蚴,以各虫株毛蚴定量感染钉螺,观察、比较各虫株对中间宿主钉螺的感染率、感染性钉螺存活率、尾蚴开放前期和产尾蚴量。结果用一条毛蚴感染一只钉螺,日本血吸虫江苏吡喹酮敏感株和抗性株对钉螺的感染率分别为8.99%和19.74%(χ~2=3.948,P=0.047),平均每螺产尾蚴量分别为1 460.2条和1 039.3条(t=2.507,P=0.02);湖南吡喹酮敏感株和抗性株对钉螺的感染率分别为10.00%和21.52%(χ~2=3.980,P=0.046),平均每螺产尾蚴量分别为1 319.4条和1 003.5条(t=2.566,P=0.017)。日本血吸虫吡喹酮敏感株和抗性株尾蚴开放前期和感染性钉螺存活率差异均无统计学意义(P均>0.05)。结论日本血吸虫吡喹酮抗性株对钉螺的易感性高于敏感株,而平均每螺产尾蚴量则低于敏感株。Objective To investigate the difference of biological characteristics between the praziquantel-resistant and-sus-ceptible isolates of Schistosoma japonicum in intermediate host Oncomelania hupensis snails. Methods Mice were infected with cercariae of praziquantel-resistant and-susceptible isolates of S. japonicum,and the parasite eggs were collected 37 days post-in-fection to hatch miracidium. Then,the snails were infected with the miracidium of each parasite isolate. The snail infection,sur-vival rate of infected snails,prepatent period of cercariae,and the total number of cercariae shed from each infected snail were observed and compared between the praziquantel-resistant and-susceptible isolates of S. japonicum. Results If each snail was exposed to a single miracidium,there were significant differences between the praziquantel-resistant and-susceptible Jiangsu isolates in the snail infection（8.99%vs. 19.74%;χ2=3.948,P=0.047）and the number of cercaria released from a single snail （1460.2 vs. 1039.3;t=2.507,P=0.02）,and there were significant differences between the praziquantel-susceptible and-re-sistant Hunan isolates in the snail infection（10.00%vs. 21.52%;χ2=3.980,P=0.046）and the number of cercaria released from a single snail（1319.4 vs. 1003.5;t=2.566,P=0.017）. However,there were no significant differences between the pra-ziquantel-resistant and-susceptible isolates of S. japonicum in the prepatent period of cercariae and the survival rate of infected snails（P〉0.05）. Conclusion The praziquantel-resistant isolate of S. japonicum has a higher susceptibility to O. hupensis but less cercaria released from each infected snail than the susceptible isolate.

关 键 词：日本血吸虫 吡喹酮 抗药性 钉螺 生物学特性 

分 类 号：R383.24[医药卫生—医学寄生虫学]