重组逆病毒pRevTRE-ETRE-E77.43转导小鼠后抗血吸虫感染作用  

Preparation of recombinant retrovirus pRevTRE-E77.43 and its protective effect in a mouse model of Schistosoma japonicum infection

作　　者：曾凡胜 罗赛群[2] 熊德慧[2] 俞远京[3] 钱颖骏[4] 秦志强[4] 

机构地区：[1]益阳市洞庭湖区血吸虫与病原生物控制技术重点实验室,益阳医学高等专科学校,益阳413002 [2]中南大学生命科学学院分子生物学研究中心 [3]中南大学湘雅医学院实验动物中心 [4]中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织热带病合作中心,科技部国家级热带病国际联合研究中心,卫生部寄生虫病原与媒介生物学重点实验室

出　　处：《中国血吸虫病防治杂志》2017年第6期730-735,共6页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金项目(30400256;31100887);湖南省科技计划项目(社会发展支撑计划)(2014SK3008;2015DK3008));湖南省教育厅科研项目(14C1125)

摘　　要：目的探讨东方田鼠E77.43基因片段治疗日本血吸虫感染的生物学功能。方法构建pRevTRE-E77.43重组逆转录病毒载体,转染PA317细胞,用Hygromycin筛选后获得稳定表达的细胞株,并进行重组逆转录病毒的包装、浓缩与纯化。通过静脉或腹腔途径将制备的重组病毒注射到日本血吸虫感染模型小鼠体内,探讨目的基因在小鼠体内的表达以及体内治疗血吸虫感染的作用。结果病毒注射动物机体7 d后目的基因就有表达,且一直能持续表达45 d以上。pRevTRE-E77.43诱导小鼠产生了31.0%的减虫率,高于pRevTRE的1.2%(t=3.524,P<0.01);pRevTRE-E77.43诱导小鼠产生了35.0%的减卵率,高于pRevTRE的0.9%(t=9.485,P<0.01)。结论 pRevTRE-E77.43能对血吸虫感染模型小鼠产生一定程度的治疗效果,说明东方田鼠E77.43基因参与了东方田鼠抗日本血吸虫感染的生物学作用过程。constructed, and recombinant retro-viral vectors were transfected into PA317 cells, and the stable cell lines were obtained by hygromycin screening, followed by the packaging, concentration and purification of recombinant retrovirus. The virus was transferred to the mice infected by S. japoni-cum via intravenous or intraperitoneal injection, through which the express of target gene and the treatment function in vivo were observed. Results The experiment showed the recombinant virus injected mice could efficiently express E77.43 on the 7th day after the injection which lasted for forty-five days thereafter. A significant reduction in adult worms （31.0%） and a high reduction （35.0%） in liver eggs were induced by pRevTRE-E77.43, while the reduction in adult worms and that in liver eggs was 1.2%and 0.9%induced by pRevTRE respectively （t=3.524, 9.485, both P〈0.01）. Conclusion pRevTRE-E77.43 could be used for the treatment of S. japonicum infection, indicating that E77.43 may involve in the natural resistance of M. fortis to S. japonicum infec-tion.

关 键 词：日本血吸虫 东方田鼠 重组逆转录病毒 抗性相关基因 减虫效果 

分 类 号：R532.21[医药卫生—内科学]