新发展晚期血吸虫病患者循环microRNAs表达谱的初步研究  

Expression profiles of circulatingmicro RNAs in newly-developed advanced schistosomiasis patients

作　　者：陈伟[1] 董晨[2] 尤璐[1] 唐凤[1] 华海涌[1] 

机构地区：[1]国家卫生计生委寄生虫病预防与控制技术重点实验室,江苏省寄生虫与媒介控制技术重点实验室,江苏省血吸虫病防治研究所,无锡214064 [2]苏州大学医学部公共卫生学院

出　　处：《中国血吸虫病防治杂志》2017年第6期788-790,806,共4页Chinese Journal of Schistosomiasis Control

基　　金：江苏省科技厅临床专项(BL2014020);江苏省"科教强卫工程"项目资助

摘　　要：目的研究新发展晚期血吸虫病(晚血)患者循环miRNAs的表达谱特征。方法选取新发展晚血患者、未达到晚血的有史经治人群和健康对照人群各10例,采用Agilent Human micro RNAs microarray Rel 12.0芯片,检测570个人类相关的循环miRNAs的表达水平,分析各组循环miRNAs的表达差异。结果与健康对照人群相比,新发展晚血患者的miRNAs有4个表达上调,16个表达下调。而在健康对照和有史经治组的血清中存在着差异表达的miRNAs共有27个,其中6个表达上调,21个表达下调。其中mi R-383在有史经治组和新发展晚血患者组中的表达分别降低了4.23倍和11.82倍。结论新发展晚血患者、有史经治者和健康人群之间循环miRNAs的表达特征存在显著差异,循环mi R-383可能与新发展晚血的发生发展有关,值得进一步研究。Objective To study the expression profiles of circulating microRNAs（miRNAs）in newly-developed advanced schistosomiasis patients. Methods Ten healthy controls（Group 1）,ten patients with schistosomiasis history（without advanced schistosomiasis,Group 2）and ten patients with newly-developed advanced schistosomiasis（Group 3）were randomly selected from Jiangsu Province. The expression profiles of 570 human related circulatingmiRNAs were measured and analyzed with the Agilent Human microRNAs microarray Rel 12.0. Results Compared to the healthy controls,four miRNAs were up-regulated, while 16 miRNAs were down-regulated in the patients with newly-developed advanced schistosomiasis. Moreover ,six miRNAs were up-regulated,while 21 miRNAs were down-regulated in the patients in Group 2. Among of them,the expression level of miR-383 was significantly decreased by 4.23 and 11.82 folds in Group 2 and Group 3,respectively. Conclusions There are significant differences among the healthy controls,patients with schistosomiasis history（without advanced schistosomiasis）and the patients with newly-developed advanced schistosomiasisin the expression profiles of circulatingmiRNAs. Moreover ,circulat-ing miR-383 might be involved in the development of newly-developed advanced schistosomiasis.

关 键 词：新发展晚期血吸虫病 循环miRNAs 差异表达 

分 类 号：R532.21[医药卫生—内科学]