HPLC法测定参麦地黄丸中马钱苷的含量  

Determination the Content of Loganin in Shenmaidihuang Pills by HPLC

作　　者：刘思飞[1] 杨晓珑 李丹慧[3] 孙立新[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳第二中学1808班,辽宁沈阳110016 [3]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《今日药学》2017年第12期819-821,共3页Pharmacy Today

摘　　要：目的建立测定参麦地黄丸中马钱苷含量的高效液相色谱(HPLC)法。方法色谱柱:Diamonsil C_(18)柱(4.6 mm×200mm,5μm),检测波长:236 nm,流速:1.0 mL·min^(-1),柱温:40℃,进样量:20μL,流动相:四氢呋喃-甲醇-乙腈-0.05%磷酸水溶液(1∶4∶8∶87,V/V)。结果马钱苷浓度在8.048~40.24μg·mL^(-1)范围内,线性关系良好,平均加样回收率为99.9%,RSD为1.1%。结论该方法准确、简便,可用于参麦地黄丸中马钱苷的含量测定。OBJECTIVE To establish a method for the determination of loganin in Shenmaidihuang Pills. METHODS The samples were analyzed on a Diamonsil Cls （4.6 mm×200 mm, 5 μm） column with the mobile phase consisted of tetrahydrofuran- methanol-acetonitrile-0.05% phosphoric acid aqueous solution （ 1 ： 4 ： 8 ： 87, V/V） at a flow rate of 1.0 mL · mi年……-1. The detection wavelength was set at 236 nm, the sample size was 20 μL, and the column temperature was 40 ~C. RESULTS The calibration curve was linear within the range of 8.048-40.24 μg·mL-1 （ r = 1.000） for loganin. The average recovery was 99.9%, and RSD was 1.1%. CONCLUSION This method is accurate and rapid, and can be used for the determination of loganin in Shenmaidihuang Pills.

关 键 词：参麦地黄丸 马钱苷 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]