脂肪来源干细胞对成纤维细胞放射性损伤的影响和蛋白组学研究  

Study of the effect of adipose-derived stem cells on radiation injury in fibroblasts and proteomics

作　　者：栗颖利[1,2] 唐炜雅 杨超[2] 邢新[2] 

机构地区：[1]济南军区总医院整形外科,山东济南250031 [2]第二军医大学长海医院整形外科,上海200433

出　　处：《中国美容整形外科杂志》2017年第12期712-715,共4页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的探讨大鼠脂肪来源干细胞(ADSCs)对放射后成纤维细胞(Fb)的影响。方法体外原代培养SD大鼠ADSCs和真皮Fb,分别对2种细胞进行鉴定,Fb辐照后对2种细胞建立共培养模型。将Fb分为Fb1、Fb2和Fb3组,对各组进行凋亡和细胞周期检测以及蛋白高通量蛋白质谱检测。结果 ADSCs高表达CD29和CD44,低表达CD31和CD45,大鼠Fb高表达波形蛋白。辐射损伤后Fb 2组凋亡(32.30±7.21)%(P<0.05,Fb2∶Fb1),Fb3组的ADSCs干预后下降到(9.80±3.08)%(P<0.05,Fb3∶Fb2)。8 Gy照射后,Fb周期阻滞在G2期(P<0.05,Fb2∶Fb1),ADSCs干预后的Fb3组Fb较Fb2组G2期减少,G1期增加,差异有统计学意义(P<0.05,Fb3∶Fb2)。结论 ADSCs干预放射后的Fb可使其凋亡降低,细胞周期进程加快,蛋白质谱检测差异蛋白富集在PI3K-AKT和MAPK通路。Objective To explore the effect of adipose-derived stem cells（ADSCs） on radiation injury in fibroblasts（Fb）.Methods The ADSCs and dermal Fb from SD rats were cultivated in vitro and identified. After radiation of Fb, a co-culture model of two kinds of cells was established. The Fb was divided into three groups： Fb1, Fb2, and Fb3. Apoptosis, cell cycle, and high throughput protein mass spectrometry detection were performed in each group. Results CD29 and CD44 expression were high, but CD31 and CD45, expression was low in ADSCs. Vimentin expression was high in Fb. After radiation injury, the apoptotic rate of fibroblasts in the Fb2 group was（32.3±7.21） %（P0.05, Fb2∶Fb1）. The apoptotic rate of fibroblasts in the Fb3 group decreased to（9.80±3.08） % after ADSCs intervention（P0.05, Fb3∶Fb2）. After irradiation of 8 Gy, the cell cycle of fibroblasts was arrested in the G2 phase（P0.05, Fb2∶Fb1）. After ADSCs intervention, the fibroblasts decreased in the G2 phase, but increased in the G1 phase of group Fb3 compared with group Fb2. The difference was statistically significant（P0.05, Fb3∶Fb2）. Conclusion ADSCs can significantly reduce the apoptosis of fibroblasts after irradiation. The cell cycle procession was accelerated. Protein mass spectrometry suggested that the differential proteins were enriched in the PI3 K-AKT and MAPK pathways.

关 键 词：脂肪干细胞 成纤维细胞 凋亡 细胞周期 蛋白组学 

分 类 号：R622[医药卫生—整形外科]