人脂肪细胞外基质的制备及评价  

Preparation and characterization of the extracellular matrix of human adipose tissue

作　　者：舒军 马超[1] 温艳玲 李金龙[3] 马恬[1] 张萌萌[1] 姜珊[1] 韩为东[4] 韩岩[1] 

机构地区：[1]解放军总医院整形修复科,北京100853 [2]大连医科大学,辽宁大连116044 [3]解放军总医院病理科,北京100853 [4]解放军总医院分子免疫室,北京100853

出　　处：《中国美容整形外科杂志》2017年第12期716-719,共4页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的探寻细胞体外生长理想的三维支架结构的。方法人脂肪细胞外基质的制备:通过物理、化学、酶处理联合的方法进行脂肪组织脱细胞处理,获得人脂肪细胞外基质成分,制备成基质材料;并对人脂肪细胞外基质材料进行评价。采用CCK-8法检测基质材料的细胞毒性,观察基质材料的体外黏附情况;采用Ki67染色来观察h ADSC-gfp在基质材料的增殖情况,观察脂肪间充质干细胞的分化功能。结果⑴脂肪组织经脱细胞处理可制备不同形状的基质材料。⑵基质材料脱细胞彻底。⑶基质材料中含丰富的胶原蛋白、层黏连蛋白等有效成分。⑷基质材料无细胞毒性;细胞在基质材料上的黏附及增殖能力强;接种基质材料上的脂肪来源干细胞可诱导向表皮细胞分化。结论人脂肪细胞外基质制作方法简单,脱细胞较彻底,有效成分保留较好,无细胞毒性,易于细胞黏附、增殖及分化。Objective To explore the ideal three-dimensional scaffold structure for cell growth in vitro. Methods Preparation of human adipose extracellular matrix： adipose tissue decellularization is performed by a combination of physical, chemical, and enzyme treatments. The human adipose extracellular matrix was obtained and lyophilized to prepare matrix material with different shapes. The human adipose extracellular matrix material was evaluated. The cytotoxicity of matrix material was detected by the CCK-8 method. The adhesion of matrix material in vitro was observed. The proliferation of h ADSC-gfp in matrix material by Ki67 staining and the differentiation of adipose-derived mesenchymal stem cells was also observed. Results（1） The adipose tissue was treated with different shapes of matrix materials by decellularization,（2） matrix material was thoroughly acellular,（3） matrix material was rich in collagen, laminin, and other active ingredients,（4） matrix material was without cytotoxicity. The adhesion and proliferation abilities of cells on the matrix materials were strong. The adipose-derived stem cells inoculated on the matrix material could be induced to the epidermal cells. Conclusion The reparation method of the extracellular matrix of human adipose tissue is simple： the cells are more completely decellularized and the effective components are well-preserved. This method yields cells that are low in density, high in porosity, with good water absorption, no cytotoxicity, and ease of cell adhesion, proliferation, and differentiation.

关 键 词：脂肪细胞外基质 脱细胞基质 脂肪组织 脂肪来源干细胞 支架材料 

分 类 号：R622[医药卫生—整形外科]