脂肪来源干细胞促进大鼠坐骨神经再生机制研究  

Study of the regenerative mechanism of adipose-derived stem cells on SD rats with sciatic nerve defects

作　　者：宋起滨[1] 王哲[2] 王宇令[1] 李杨[1] 林爽[1] 

机构地区：[1]中国医科大学附属盛京医院整形科,辽宁沈阳110004 [2]中国医科大学附属盛京医院病理科,辽宁沈阳110004

出　　处：《中国美容整形外科杂志》2017年第12期720-722,共3页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：辽宁省博士启动基金(20131142);辽宁省教育厅科学技术研究项目(LK201602)

摘　　要：目的探讨高分子神经套管负载大鼠自体脂肪源性干细胞(ADSCs)对大鼠坐骨神经损伤修复的作用。方法提取10只SD大鼠腹股沟区ADSCs作为实验组,另选择10只SD大鼠作为对照组,制作单侧坐骨神经5.0 mm缺损模型。术后12周应用神经电生理检测,腓肠肌湿重和组织学观察,分析评价神经再生效果。结果术后12周,实验组存活7只,对照组存活8只,实验组坐骨神经传导速率为(12.39±0.71)ms,对照组为(8.13±0.87)ms,其差异有统计学意义(P<0.05);腓肠肌湿重比率实验组为47.69±0.24,对照组为35.77±0.73,其差异有统计学意义(P<0.05)。对照组有髓神经纤维数量较少,呈散在分布;实验组有髓神经纤维数量较多,有神经束膜形成。结论 ADSCs对于SD大鼠周围神经生长有促进作用。Objective To explore the efficacy of the application of poly lactic co glycolic acid（PLGA） nerve tube loaded with adipose-derived stem cells in the repair of sciatic nerve defects in SD rats. Methods The adipose-derived stem cells were extracted from 10 SD rats as an experimental group. Another 10 SD rats were selected as the control group. A 5.0 mm defect model of the unilateral sciatic nerve of SD rats was made. Twelve weeks after operation, the effect of nerve regeneration was evaluated through electroneurophysiological examination, histological observation, and gastrocnemius wet weight. Results After 12 weeks, 7 rats from the experimental group and 8 rats from the control group survived. The sciatic nerve conduction rate was（12.39±0.71） ms in the experimental group, and（8.13±0.87） ms in the control group. The wet weight ratio of the gastrocnemius muscle was（47.69±0.24） in the experimental group and（35.77±0.73） in the control group. All differences were statistically significant. The number of myelinated nerve fibers were less and scattered in the control group. A larger number of myelinated nerve fibers and nerve fascicles formed in the experimental group. Conclusion ADSCs can promote the growth of the peripheral nerve in SD rats.

关 键 词：脂肪源性干细胞 SD大鼠 周围神经缺损模型 PLGA神经套管 

分 类 号：R622[医药卫生—整形外科]