雷帕霉素调控光老化成纤维细胞MMPs和COL-1表达  

Regulation of rapamycin on the expression of MMPs and COL-1 in photo-aging fibroblasts

作　　者：秦登科 贾传龙 卢勇舟 杨清建 陈亮 吴心愿 任润健 朱晶晶 郭妤 杨平 周轶群 毕波 朱宁文 刘天一 

机构地区：[1]复旦大学附属华东医院整形外科,上海200040 [2]复旦大学附属华东医院皮肤科,上海200040

出　　处：《中国美容整形外科杂志》2017年第12期723-725,732,共4页Chinese Journal of Aesthetic and Plastic Surgery

基　　金：国家自然科学基金(81272125)

摘　　要：目的探讨雷帕霉素(RAPA)对光老化皮肤成纤维细胞(FBs)基质金属蛋白酶(MMPs)表达和Ⅰ型胶原蛋白(collagen-Ⅰ)合成的影响。方法采用cck-8法检测RAPA对FBs活性的影响,甄选最佳应用浓度,利用UVB体外反复照射构建成纤维细胞的老化模型,RAPA预处理细胞,造模结束24 h后,实时荧光定量检测MMPs的表达;造模结束48 h,蛋白质印迹法检测Ⅰ型胶原的表达。结果低剂量RAPA对FBs的细胞活性无影响,5μm/L的RAPA作为后续细胞处理的药物浓度为佳。UVB组的光老化FBs中,MMP-1、2、9的表达均显著升高;而RAPA预处理后能明显降低UVB引起的MMPs过度分泌;同时使Ⅰ型胶原的表达量增多。结论 RAPA可降低光老化FBs中MMPs的表达,增加Ⅰ型胶原的合成,维持光老化皮肤ECM成分的稳定,在一定程度上缓解细胞光老化程度。Objective To investigate the influence of rapamycin（RAPA） on the expression of matrix metalloproteinases（MMPs）and the synthesis of type I collagen（collagen-I） in photo-aging skin fibroblasts（FBs）. Methods The best concentration was selected by the effect of RAPA on the proliferative activity of FBs tested by cell counting kit-8（CCK-8） assay. The photo-aging model of FBs was structured by repeated UVB radiation. The cells were pretreated with RAPA. The influence of RAPA on the expression of MMPs of photo-aging skin FBs was detected by real-time fluorescence quantitative PCR at 24 hours after modeling and the synthesis of collagen I was detected by western blotting at 48 h after modeling. Results The proliferative activity of fibroblasts was not affected by RAPA in a low dose manner, 5 μm/L was the best concentration for the subsequent treatment. The UVB irradiation increased the expressions of MMP-1, 2, 9 of FBs. RAPA pretreatment significantly decreased expression of MMPs caused by UVB and increased the expression of collagen I.Conclusion RAPA can maintain the metabolic balance of dermal ECM by decreasing the expression of MMPs and increasing the synthesis of collagen I in photo-aging FBs, and to a certain extent, alleviate the degree of cell photoaging.

关 键 词：成纤维细胞 雷帕霉素 光老化 基质金属蛋白酶 Ⅰ型胶原 

分 类 号：R622[医药卫生—整形外科]