植物中指导RNA偶联转录调控因子抑制基因表达研究  

Down-regulation of Gene Expression by Guided and Tethered Regulator(GRATR) in Plant

作　　者：刘营 刘国富[1] 鲍岳 孟祥潮 张俊华 曹雪松 

机构地区：[1]聊城大学,聊城252059

出　　处：《基因组学与应用生物学》2017年第12期5232-5237,共6页Genomics and Applied Biology

基　　金：国家自然科学基金项目"基于CRISPR/Cas系统的新型植物基因组编辑技术研究"(No:31370387)资助

摘　　要：本研究建立了一种简单、精确和高效的新型抑制植物基因表达的技术体系。将绿色荧光蛋白(green fluorescent protein,GFP)、指导RNA(guide RNA,g RNA)和编码转录抑制因子与噬菌体外壳融合蛋白的三种基因构建到植物表达载体中。利用农杆菌介导的植物瞬时表达技术侵染本氏烟草。研究显示gfp基因在烟草叶片中的表达受到显著抑制,抑制率达到36.2%。此系统可运用于对其他基因进行基因调控,由于只需要改变g RNA,而其他组成原件保持不变,因此该技术具有操作简单及广泛的适应性等特点。为在植物基因组中精确抑制基因表达提供了有效的工具。This study established a simple, accurate and efficient technology system for inhibiting plant gene expression. The genes of green fluorescent protein, guide RNA and fusion protein of transcription inhibitory factor and MS2 phage coat protein were constructed into the plant expression vector. Transient expression was implemented by using Agrobaterium infiltration on the leaves of Nicotiana benthamiana. The results showed that the expression of gfp gene in the leaves were significantly suppressed and the inhibitory rate was 36.2%. This system was expected to be applied in regulating other genes’ transcription. Because only g RNA was needed to change, while other component remained the same, our system possessed the characteristics of simple operation and extensive adaptability. It might provide an effective tool for inhibiting gene expression in plant.

关 键 词：转录调控因子 指导RNA基因 噬菌体MS2外壳蛋白 转录抑制子 

分 类 号：Q943.2[生物学—植物学]