熊果酸对Hacat细胞增殖及凋亡的影响  

Effect of Ursolic Acid on the Proliferation and Apoptosis of Hacat Cells

作　　者：王俊慧[1] 李敏[2] 阚杰[2] 刘瓦利[1] 

机构地区：[1]中国中医科学院广安门医院皮肤科,北京100053 [2]中国中医科学院广安门医院分子生物实验室,北京100053

出　　处：《中国中医基础医学杂志》2017年第12期1714-1717,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(81403398)-清热活血解毒法对银屑病相关神经内分泌免疫网络节点-神经生长因子(NGF)及其受体系统的调控研究

摘　　要：目的:观察中药白花蛇舌草主要有效单体熊果酸对人永生化细胞株Hacat细胞增殖及凋亡的影响,明确百花蛇舌草治疗银屑病的机制。方法:体外培养人角质形成细胞株Hacat,使用WST-8法检测熊果酸对Hacat细胞增殖的影响,采用流式细胞仪检测熊果酸对Hacat细胞细胞周期及凋亡的影响,并采用免疫荧光染色的方法标记凋亡细胞,从而进一步验证熊果酸对Hacat细胞凋亡的影响。结果:熊果酸能有效抑制Hacat细胞的增殖,与药物浓度呈正相关,同时可以将Hacat细胞阻滞于G1/G0期,同时可促进Hacat细胞凋亡。结论:熊果酸对角质形成细胞增殖有抑制作用,角质形成细胞凋亡有促进作用,推断其可能是白花蛇舌草治疗银屑病的有效单体之一。Objective： To identify the effects of Ursolic Acid on the proliferation and apoptosis of Hacat ceils. Methods： Haeat cells Were cultured by human eutin. The proliferation of Hacat cells was detected by WST-8. The cell cycle and apoptosis of Haeat cells were detected by flow eytometry and immunofluorescence assay. Results ： Ursolic Acid inhibited the proliferation of Hacat cells, and blocked the cell cycle in G1/G0 phase. Ursolie Acid induced the apoptosis of Hacat cells. Conclusion： Ursoilc Acid can inhibit the proliferation and promote the apoptosis of Hacat cells, consequently which may be one of potential effective monomers of Herba Hedyotis for treating psoriasis.

关 键 词：熊果酸 HACAT细胞 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]