高温高湿和低温环境对存放全血质量的影响  

Effect of temperature and humidity on blood quality of whole blood of emergency blood collection

作　　者：马平[1] 张艳宇[1] 单桂秋[2] 沈建军 周谋[2] 邓江[1] 肖军[1] 周锡鹏[1] 许金波[1] 吕丽萍[1] 

机构地区：[1]军事医学科学院野战输血研究所,全军血液安全与保障重点实验室,血液安全保障技术研究北京市重点实验室,北京100850 [2]广州军区广州总医院输血科,广州510010 [3]解放军211医院输血科,哈尔滨150080

出　　处：《军事医学》2017年第9期735-738,共4页Military Medical Sciences

摘　　要：目的研究高温、高湿及低温严寒等环境因素对存放全血质量的影响。方法以现场实验的方式,共采集9名献血者的新鲜全血,平均分成40份,每组20份分别在高温高湿区的3个模拟采血点放置0、3、6、12和24 h,低温严寒区的4个模拟采血点放置0、1、2和3 h后,测定血浆游离血红蛋白(FHb)浓度、血浆凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)和纤维蛋白原(FIB)。结果高温(27~37℃)高湿(60%~90%RH)环境下放置24 h的新鲜全血,FHb、APTT、PT、TT和FIB与对照组相比无明显差异。低温(0~13℃组和-18^-20℃组)对全血的血液质量产生影响,在无加温装置的帐篷中,全血放置1 h后出现冰冻;在有加温装置的帐篷中,全血中FHb浓度随时间的延长逐渐增高,放置2 h后其血浆中的FHb浓度明显高于对照组,但APTT、PT、TT和FIB与对照组相比差异无统计学意义。结论在高温高湿条件下,室温下放置24 h的全血质量未见明显改变;在低温严寒的条件下,在具有加温设施的帐篷中,室温下放置1 h的全血质量未见明显改变。Objective To study the effects of environmental factors,including high temperature,high humidity and low temprtature,on the quality of whole blood.Methods Fresh blood was collected from 9 blood donors and divided into 40 parts.One group that comprised 20 samples was used to assess the effects of storage at high temperature and high humidity（0,3,6,12 and 24 h）,and the other group（20 samples） was used to determine the effects of low temprtature（0,1,2 and 3 h）.The serum free hemoglobin（FHb） concentration,plasma prothrombin time（PT）,activated partial thromboplastin time（APTT）,thrombin time（TT） and fibrinogen（FIB） were measured and analyzed.Results The high temperature（27-37℃） and humidity（60%-90% RH） had no effect on FHb,PT,APTT,TT or FIB of whole blood.The low temperature（0-13℃ and-18--20℃） had some effect on the quality of whole blood.The FHb of test group was higher than that of control group.There was no significant difference in PT,APTT,TT and FIB between the two groups.Conclusion Under high temperature and humidity conditions,24 hours of whole blood placement is feasible.Under low temperature or cold conditions,short-term whole blood placement is feasible in a tent with heating facilities.

关 键 词：高温 高湿 低温 血液保存 凝血酶原时间 纤维蛋白原 

分 类 号：R82[医药卫生—临床医学]