长航对舰载保存去白细胞悬浮红细胞生化指标的影响  

Biochemical indices of red blood cells in additive solution leukocytes reduced preserved in navy ship force during a long voyage

作　　者：史大川 姚雪[1] 石磊[1] 李燕[1] 刘伟[1] 孙伟杰[1] 韩颖[2] 刘敏霞 王东根[2] 郑长青[1] 

机构地区：[1]解放军401医院输血科,山东青岛266071 [2]军事医学科学院野战输血研究所,全军血液安全与保障重点实验室,血液安全保障技术研究北京市重点实验室,北京100850

出　　处：《军事医学》2017年第9期745-748,751,共5页Military Medical Sciences

摘　　要：目的探讨和研究海上航行对舰载保存去白细胞悬浮红细胞生化指标的影响与变化。方法根据《献血者健康检查要求》(GB 18467-2011),于出海前1 d,随机采集和制备去白细胞悬浮红细胞10人份(2 U)。每份血液等分为两份,标记为对照组和试验组,分别于长航第1、3、5、7、14、21天取样进行生化指标检测。结果 (1)总蛋白(P=0.235)和白蛋白(P=0.119)浓度随保存时间的延长变化不明显;两组的总蛋白(P=0.825)和白蛋白(P=0.834)浓度差异无统计学意义。(2)肌酐(P=0.001)和尿酸(P=0.001)浓度随保存时间的延长变化比较显著;两组肌酐(P=0.643)和尿酸(P=0.923)浓度差异无统计学意义。(3)总胆固醇浓度随保存时间的延长变化不明显(P=0.354),而甘油三酯浓度随保存时间的延长变化比较显著(P=0.005);两组的总胆固醇(P=0.959)和甘油三酯(P=0.910)浓度差异无统计学意义。(4)乳酸脱氢酶(P<0.001)、羟丁酸脱氢酶(P<0.001)和肌酸激酶浓度(P<0.001)随保存时间的延长而逐渐升高;两组乳酸脱氢酶(P=0.759)、羟丁酸脱氢酶(P=0.528)和甘油三酯(P=0.918)浓度差异无统计学意义。(5)分组效应与时间效应的交互作用对渗透压(P=0.968)和葡萄糖浓度(P=0.406)影响不明显,两组的渗透压(P=0.569)和葡萄糖浓度(P=0.115)差异无统计学意义。结论在4~5级海况条件下,长航对去白细胞悬浮红细胞的储存具有一定的影响,但与常规储血冰箱(4±2)℃保存的主要生化指标结果变化仍比较相近。该研究结果对进一步研究海上血液保障具有重要的临床意义。Objective To explore the influence on and changes in the biochemical indices of red blood cells（RBCs） in additive solution leukocytes reduced preserved in navy ship force on a long voyage.Methods According to the Requirement of Health Examination for Blood Donors（GB 18467-2011）,RBCs in additive solution leukocytes reduced were prepared from 10 healthy voluntary blood donators one day before sailing.Each blood sample was divided into two parts,one in test group and another in control group.All the groups had samples taken for the biochemical index detection after 1,3,5,7,14 and 21 days of sailing respectively.Results（1）The change in total protein（P = 0.235） and albumin（P = 0.119） concentration was not obvious,and the difference between the two groups was not significant.（2）The change in total creatinine（P = 0.001） and uric acid（P = 0.001） concentration was obvious,but the difference between the two groups was not significant.（3）The change in total cholesterol（P = 0.354） concentration was not obvious,but the change in triglycerides（P = 0.005） concentration was significant.The difference between the two groups was not significant.（4）The concentration of lactate dehydrogenase,hydroxybutyrate dehydrogenase and creatine kinase increased with the time of preservation（P〈0.001）.The difference between the two groups was not significant.（5）The interaction between grouping effect and time effect had no significant influence on the concentration of osmolarity（OSM）（P = 0.968） and glucose（Glu）（P = 0.406）.Between the two groups,the difference of concentrations of OSM（P = 0.569） and Glu（P = 0.115） was not significant.Conclusion Under the 4 class sea conditions,a long voyage has some impact on the storage of RBCs in additive solution leukocytes reduced,as in the conventional blood storage refrigerator（4 ± 2） ℃.The results of this study have important clinical implications for our further study of marine blood support.

关 键 词：长航 去白细胞悬浮红细胞 生化指标 舰载保存 血液保存 红细胞 运输 

分 类 号：R82[医药卫生—临床医学]