水稻Bph14基因生理功能初探  

Preliminary Study on Physiological Function of Gene Bph14 in Rice

作　　者：李三和[1,2] 闸雯俊 陈志军[1,2] 周雷[1] 刘凯 游艾青[1,2] 

机构地区：[1]湖北省农业科学院粮食作物研究所粮食作物种质创新与遗传改良湖北省重点实验室,武汉430064 [2]主要粮食作物产业化湖北省协同创新中心(长江大学),荆州434025

出　　处：《生物技术通报》2017年第12期93-98,共6页Biotechnology Bulletin

基　　金：国家转基因重大专项(2014ZX0800101B);国家科技支撑计划(2015BAD01B01)

摘　　要：Bph14基因是第一个被克隆的水稻抗虫基因,但鲜有Bph14基因对水稻生理方面影响的报道。研究该基因对水稻生理方面的影响有助于全面理解Bph14基因对褐飞虱的抗性机制。将Bph14基因转入水稻栽培品种中,对携带有外源基因的转基因株系通过荧光定量PCR检测乙烯合成和非生物胁迫相关基因的表达,另外,分别用5μmol/L ABA和250 mmol/L Na Cl处理转基因水稻株系和相应受体材料,结果表明,与乙烯合成相关的基因Os ACO2,Os ACS2,Os ACS6,O1g P5CS和O5g P5CS的表达量均较转基因受体m5274发生了变化,与干旱、耐盐等非生物胁迫相关的基因Cat A、Cat B、Cat C、RAB16A、LEA3、LIP9、Sal T、Adh I等也都不同于在受体m5274中的表达。同时Bph14基因的导入也提高了水稻对ABA的敏感性和对盐胁迫的耐受性,说明Bph14基因在水稻中可能参与调控乙烯合成以及非生物胁迫应答的功能基因的表达。Gene Bph14 is the first cloned rice insect-resistant gene,however,there were few reports about the effects of Bph14 gene on rice physiology. The study of the physiological effects of the gene on rice would contributes to a comprehensive understanding of the resistance mechanism of gene Bph14 to brown planthopper. The gene Bph14 was transferred into rice cultivar m5274,and genes related to ethylene biosynthesis and abiotic stress response were detected by Q-PCR in transgenic lines carrying gene Bph14. In addition,transgenic lines and the accepter line were treated with 5 μmol/L ABA and 250 mmol/L NaCl respectively. In these lines,OsACO2,OsACS2,OsACS6,O1gP5CS, and O5gP5CS expressed differently from those in m5274. Genes such as CatA,CatB,CatC,RAB16A,LEA3,LIP9,SalT,and AdhI related to abiotic stress of drought and salt-resistance also differently expressed from those in m5274. At the same time,the introduction of Bph14 gene improved the sensitivity of rice to ABA and the resistance to salt stress. These results indicate that gene Bph14 in rice probably participates in the regulation of ethylene biosynthesis and expression of functional genes in abiotic stress response.

关 键 词：Bph14基因 乙烯合成 非生物胁迫 水稻 

分 类 号：S511[农业科学—作物学]