转Bt棉主茎叶Cry1Ab/c蛋白含量的时空分布分析  

Tempo-spatial Distribution of Cry1Ab/c Protein in the Main Stem Leaves of Transgenic Bt Cotton

作　　者：肖海兵[1,2] 王鹏军 李先锋[3] 董红强 杨明禄[1,2] 

机构地区：[1]塔里木大学植物科学学院,阿拉尔843300 [2]农业部阿拉尔作物有害生物科学观测站,阿拉尔843300 [3]新疆生产建设兵团第一师阿拉尔农场,阿拉尔843300

出　　处：《生物技术通报》2017年第12期108-111,共4页Biotechnology Bulletin

基　　金：科技支疆计划项目(2014AB009);塔里木大学校长基金青年项目(TDZKQN201601)

摘　　要：为明确M-49和X-37转基因棉Cry1Ab/c蛋白在主茎叶片的时空分布,采用酶联免疫法(ELISA)对主茎叶片的Cry1Ab/c蛋白含量进行检测,并利用考马斯亮蓝法检测叶片水溶性总蛋白含量。结果表明:X-37主茎叶片Cry1Ab/c蛋白整体含量高于M-49;两种转Bt棉主茎叶的Cry1Ab/c蛋白含量由顶端向底层逐渐增加,现蕾期(6月3日)和初花期(6月27日)底层叶片Cry1Ab/c蛋白含量显著高于顶端叶片;花铃期(8月6日)和铃期(8月26日)中上部叶片Cry1Ab/c蛋白含量均无明显差异。另外,在初花期(6月27日)主茎上部叶片的Cry1Ab/c蛋白与水溶性总蛋白比显著高于中下部叶片。总体上,两种转Bt棉主茎叶片Cry1Ab/c蛋白叶片含量分布由下至上逐渐减少,随生育期的推进呈先增后降,在初花期的上部叶片Cry1Ab/c蛋白与水溶总性蛋白比变化较其他生育期明显。The objective of this study is to reveal the tempo-spatial distribution of Cry1Ab/c protein in main stem leaves of transgenic cotton variety M-49 and X-37. The Cry1Ab/c protein content and the water-soluble protein in the main stem leaves from transgenic Bt cotton were detected by enzyme linked immunoassay（ELISA）and Coomassie brilliant blue method,respectively. The results showed that the content of Cry1Ab/c protein in the main stem leaves of the cotton variety X-37 were greatly higher than that in M-49. The contents of Cry1Ab/c protein in both varieties increased gradually from the top leaves to the bottom leaves. The Cry1Ab/c protein content of the bottom leaves were greatly higher than that of the top leaves at squaring period（3 June 2016）and initial flowering stage（27 June 2016）（P 〈 0.05）,but there were no significant differences on the content of Cry1Ab/c protein between the upper and middle parts of the main stems at blossoming and boll forming stage（6 August 2016）and boll period（26 August 262016）. Besides,the proportion of the Cry1Ab/c protein content to the water-soluble protein content in the upper leaves were greatly higher than that in the middle and lower leaves in main stem of transgenic Bt cotton（27 June 2016）. In summary,the content of Cry1Ab/c protein in the main stem leaves of both varieties decreased gradually from the bottom leaves to the top leaves,and the trend of the content of Cry1Ab/c protein lowered after increased with its growth period going on,and the ratio of the Cry1Ab/c protein to the water-soluble protein content from the upper leaves changed more significantly at initial flowering stage than other developmental stages.

关 键 词：转Bt棉 Cry1Ab/c蛋白 酶联免疫 时空分布 水溶性总蛋白 

分 类 号：S562[农业科学—作物学]