金属离子对中等嗜热混合菌活性的影响  

Effects of Metal Ions on Activities of Mixed Moderately Thermophilic Bacteria

作　　者：赵雪淞[1] 王冬旭 刘鑫[1] 石倩倩[1] 史帅 

机构地区：[1]辽宁工程技术大学辽宁省矿物加工与利用重点实验室,阜新123000

出　　处：《生物技术通报》2017年第12期151-155,共5页Biotechnology Bulletin

基　　金：国家自然科学基金项目(51204091)

摘　　要：通过金属耐受试验,测定了金属离子Mo^(6+)、Cu^(2+)、Zn^(2+)对中等嗜热混合菌(喜温嗜酸硫杆菌和氧化亚铁微螺菌共培养体系)活性的影响。结果表明,随着金属离子浓度的提高,细菌的活性降低,受试菌对上述3种金属离子的耐受浓度分别为0.6g/L、10 g/L和30 g/L,3种金属离子的毒性顺序为:Mo^(6+)>Cu^(2+)>Zn^(2+)。喜温嗜酸硫杆菌对金属离子的耐受能力高于氧化亚铁微螺菌。结果说明喜温嗜酸硫杆菌和氧化亚铁微螺菌共培养体系具有较强的金属离子耐受能力,特别是耐受钼的能力很强,适于含钼硫化矿、含钼废料以及含钼污泥等的生物浸出。The effects of metal ions Mo6＋,Cu2＋,and Zn2＋ on the activities of mixed moderately thermophilic bacteria such as Leptospirillum ferriphilum and Acidithiobacillus caldus,were tested by the metal tolerance experiment. As results,the activities of mix moderately thermophilic bacteria decreased with increasing concentration of metal ions. The tolerated concentration of bacteria tested for metal ions Mo6＋,Cu2＋ and Zn2＋ were 0.6 g/L,10 g/L and 30 g/L respectively. The toxicity order of metal ions was in Mo6＋ 〉Cu2＋〉Zn2＋. A. caldus presented a higher degree of resistance to metal ions than L. ferriphilum. Results in this study demonstrated that L. ferriphilum and A. caldus in co-culture had strong resistance to metal ions,especially to Mo6＋,thus it can be applied to the bioleaching for molybdenum sulphide ore, molybdenum waste and molybdenum sludge.

关 键 词：金属离子 中等嗜热菌 喜温嗜酸硫杆菌 氧化亚铁微螺菌 活性 

分 类 号：Q93[生物学—微生物学]