一种全自动血型分析仪加样量的校准  

Calibration for one type of sampling system of automated cross matching and blood typing analyzers

作　　者：杨新革[1] 刘持翔 孔文兵[1] 吕飘 赵晖[1] 苏建尤 苏明[1] 郭捷 周华友[1] 

机构地区：[1]南方医科大学南方医院输血科,广东广州510515 [2]北京市斑珀斯技贸有限责任公司

出　　处：《中国输血杂志》2017年第11期1231-1233,共3页Chinese Journal of Blood Transfusion

基　　金：国家高技术研究发展计划(863计划)(2013AA020201)

摘　　要：目的对Erytra全自动血型分析仪加样系统加样量的准确性进行测试及校准。方法采用称量法,应用仪器自带程序软件设定1、2号加样针的加样量,分别为10μL、25μL、50μL,按照设定好的加样量依次向DG Gel卡中加入生理盐水。用分度值为0.1 mg的电子天平,称量并记录加样前后DG Gel卡重量;所得数据用SPSS17.0进行分析,自动换算出均值(x)、标准差(SD)、变异系数(CV)、相对平均偏差(RSD);对照相关标准,根据测量计算结果,对仪器加样系统进行校准。结果 1号加样针,10μL:x10.035 0,CV 8.320 3%,RSD 0.400 2%;25μL:x24.470 0,CV 3.827%,RSD-2.071 1%;50μL:x49.765 0,CV 0.646 3%,RSD-0.420 2%;各量程均符合标准要求。2号加样针,10μL:x11.770 0,CV 10.336 4%,RSD 17.758 8%;25μL:x24.070 0,CV 5.161 1%,RSD-3.671 9%;50μL:x,49.095 0,CV 1.278 1%,RSD-0.140 1%。由数据得出,2号加样针10μL、25μL加样量存在明显偏差。分析原因,对加样系统进行维护,对2号加样针,10μL、25μL量程参数重新校准后进行测试:10μL:x10.665 0,CV 8.648 8%,RSD 6.703 3%;25μL:x25.200 0,CV 3.190 2%,RSD 0.850 4%,已符合标准要求。结论该校准试验具有可操作性,能很好的发现仪器加样误差并及时校准,能为自动化加样系统加样量的准确性提供实验数据支持,从而保证加样针加样量的准确性。Objective To examine and calibrate the sampling system of automated cross matching and blood typing analyzer. Methods Weighing method was applied,using an electronic balance with a minimum scale division of 0. 1 mg. The normal saline was used as sample to be added at the DG Gel cards,and the sampling volumes of 10 μL,25 μL,50 μL for both probe 1 and 2 were set by the analyzer＇s software. Measuring the weight of DG Gel cards before and after sampling by the electronic balance. Statistical analyses were performed by SPSS 17. 0. Mean value（ x）,standard deviation（ SD）,coefficient of variation（ CV） and relative standard deviation（ RSD） were calculated separately,according to which the sampling system was calibrated. Results For sample probe 1,the xvalue of 10 μL,25 μL and 50 μL volume were 10. 035 0,24. 470 0 and49. 765 0,respectively; the CV value of 10 μL,25 μL and 50 μL volume were 8. 320 3%,3. 827% and 0. 646 3%,respectively; the RSD value of 10 μL,25 μL and 50 μL volume were 0. 400 2%,-2. 071 1% and-0. 420 2%,respectively. For sample probe 2,the xvalue of 10 μL,25 μL and 50 μL volume were 11. 770 0,24. 070 0 and 49. 095 0,respectively; the CV value of 10 μL,25 μL and 50 μL volume were 10. 336 4%,5. 161 1% and 1. 278 1%,respectively; the RSD value of10 μL,25 μL and 50 μL volume were 17. 758 8%,-3. 671 9% and-0. 140 1%,respectively. The sample probe 1 conformed to the standard. Satisfactory results of the sample probe 2 were obtained after maintaining,with the xvalue of 10 μL and25 μL volume were and 10. 665 0 and 25. 200 0,respectively;the CV value of 10 μL and 25 μL volume were 8. 648 8% and3. 190 2%,respectively. Conclusion The calibration assay is a feasible approach that can be adopted as a routine calibration method to improve the accuracy of blood grouping and cross matching.

关 键 词：全自动血型分析仪 加样量 量程 校准 

分 类 号：R457.11[医药卫生—治疗学]