产生抗-D的RhD(-)孕妇RHD基因型分析  

RHD genotyping on anti-D positive gravidas

作　　者：蒋敏[1] 尹倩雯 李勇[1] 张健[1] 王雪明[1] 

机构地区：[1]苏州大学附属第一医院输血科,江苏苏州215006

出　　处：《中国输血杂志》2017年第11期1246-1248,共3页Chinese Journal of Blood Transfusion

摘　　要：目的了解Rh D(-)孕妇抗-D产生情况,分析产生抗体的Rh D(-)孕妇其基因型与抗-D效价之间的关系。方法收集本院2012年1月-2015年3月门诊及住院患者中血清学初筛为Rh D(-)的血液样本共559例,其中孕妇123例,均进行抗体筛检,阳性患者鉴定抗体,对发现的抗-D测定效价。对8例产生抗-D的确证Rh D(-)孕妇血清进行RHD基因型分析。结果 8例产生抗-D的孕妇中,抗-D效价为2的1例,效价32的1例,效价128的4例,效价256的1例,效价4 096的1例。其中RHD(-)2例,RHD-CE(2-9)-D 3例,Del RHD1227A 2例,DVtype2[DVa(Hus)]1例。结论 Rh D(-)孕妇中有一定比例的抗-D产生,Rh D(-)孕妇的抗-D产生及效价与RHD基因型有一定的相关性。Objective To investigate the titers of anti-D in RHD-negative pregnant women and its potential connection to RHD genotypes. Methods 559 RHD-negative serum samples from were collected and 123 of which were acquired from gravidas. We then performed confirmation tests on all serum samples to detect the presence and titers of anti-D. RHD Genotyping were further performed on 8 samples from RHD-negative pregnant women with anti-D presence. Results As for the 8 samples,the anti-D titers are： 2（ 1 samples）,32（ 1 samples）,128（ 4 samples）,256（ 1 samples） and 4 096（ 1 samples）. The genotyping results showed 2 RHD-,3 RHD-CE（ 2-9）-D,2 DelRHD1227 A and 1 DVtype2 [DVa（ Hus） ]. Conclusion Pregnancy is relevant to the production of anti-D in the RHD-negative pollution. The titer of antibodies presents certain relation to the genotype of RHD.

关 键 词：RHD(-) 抗体效价 基因分型 孕妇 

分 类 号：R457.11[医药卫生—治疗学]