甘蓝型油菜带节子叶再生体系的建立及遗传转化应用  

Establishment of Regeneration System of Cotyledon with Cotyledonary Node from Brassica napus and Transformation Application

作　　者：廖志强[1] 王小武[1] 孙亮[1] 魏志英[1] 

机构地区：[1]江西省宜春市农业科学研究所,江西宜春336000

出　　处：《中国农学通报》2017年第34期40-45,共6页Chinese Agricultural Science Bulletin

基　　金：江西省优势科技创新团队建设计划项目"双季稻区油菜早熟品种选育和机械栽培研究与应用"(20152BCB24013)

摘　　要：优良再生体系的建立是农杆菌介导植物遗传转化的基础。本研究建立以甘蓝型油菜含部分子叶节的子叶为外植体的再生体系,该再生体系为:切取5日苗龄甘蓝型油菜含部分子叶节的带柄子叶为外植体,置于添加3 mg/L6-BA的MS基本培养基中培养5天,外植体再生出丛生不定芽,不定芽再生率为100%。基于新建立的带节子叶再生体系,通过农杆菌介导,成功地将用p FGC5941载体构建的Bn TFL1基因干扰载体转入甘蓝型油菜中,从播种到得到生根抗性苗,整个转化周期只需要60~70天,较传统甘蓝型油菜以不带节子叶为外植体100~130天的转化周期大大缩短。The establishment of a good regeneration system is the foundation of agrobacterium-mediatedgenetic transformation. With cotyledons including part cotyledonary node from Brassica napus as explants, aregeneration system was established. The content of this system was that cotyledons including partcotyledonary node from five days age of rapeseed seedlings were cut as explant and then were cultured in theMS media supplemented with 3 mg/L 6-BA, and five days later, shoot regenerated from the explant. Thefrequency of shoot regeneration was 100%. Based on the new regeneration system of cotyledon withcotyledonary node, a Bn TFL1 RNAi vector constructed from p FGC5941 vector was transformed into Brassicanapus successfully by agrobacterium mediation in the research. The whole transformation period from seedingto getting rooted resistant seedlings was 60-70 days. Compared with 100-130 days period of traditionaltransformation based on regeneration culture of cotyledon without cotyledonary node, this new transformationmethod obviously shortened transformation period.

关 键 词：甘蓝型油菜 子叶节 子叶 再生体系 遗传转化 

分 类 号：Q813.12[生物学—生物工程]