小麦SSR指纹图谱及品种身份证的构建——基于毛细管电泳分析  

Construction of Wheat Variety SSR Fingerprint and ID: Based on Capillary Electrophoresis

作　　者：秦瑞英 许学 张立平 李莉[1] 方钰 汪秀峰[1] 倪金龙[1] 陆徐忠[1] 杨剑波[1] 

机构地区：[1]安徽省农业科学院水稻研究所,合肥230031 [2]安徽省农业科学院农业经济与信息研究所,合肥230031

出　　处：《中国农学通报》2017年第34期46-55,共10页Chinese Agricultural Science Bulletin

基　　金：安徽省科技重大专项计划项目"基于在线检测和物联网的农作物种子质量监管和服务关键技术研究与应用"(15czz03117)

摘　　要：为解决小麦品种区分难的问题,以黄淮海和长江中下游地区160个小麦主栽品种为试验材料,经初筛和复筛,从分布于小麦21个连锁群的105对SSR引物中,筛选出21对稳定、多态性高的引物(每条染色体上各选取1对)。对供试小麦品种进行SSR荧光标记的毛细管电泳分析,获取其SSR分子指纹信息,并与品种基本商品信息结合,进行数字化编码,构建了160个小麦的品种身份证,实现小麦品种信息唯一性、通用性、可识别性和可追溯性的统一,为小麦种子的质量追溯和管理提供便利,同时为种子市场的知识产权保护提供科学依据。This study aims to solve the problem of distinguishing wheat varieties. 160 main cultivated wheatvarieties were taken as experimental materials from Huang-Huai-Hai region and the middle-to-lower reachesof the Yangtze River, 21 pairs of stable and high polymorphism primers on each chromosome were screenedfrom 21 linkage groups distributed in 105 pairs of SSR primers as SSR fluorescence based on capillaryelectrophoresis analysis. The authors constructed the wheat variety fingerprints and digitally encodedmolecular ID of 160 wheat varieties combining with the varieties＇ basic commodity information. The resultsrealize the unity of the uniqueness, universality, identifiability and traceability of wheat varieties＇ information,which provide convenience for the quality tracing and management of wheat seeds and scientific evidence forthe protection of intellectual property right.

关 键 词：小麦品种 品种身份证 SSR荧光标记 毛细管电泳 溯源管理 

分 类 号：S512.3[农业科学—作物学]