泰州市人民医院2016年血培养阳性病原菌的分布及其耐药性  

Distribution and resistance of pathogens isolated from blood culture of Taizhou People's Hospital in 2016

作　　者：徐娟[1] 陆桃红[1] 张庆芳[1] 周成林[1] 顾兵 

机构地区：[1]泰州市人民医院检验科,江苏泰州225300 [2]徐州医科大学医学技术学院,江苏徐州221000

出　　处：《临床与病理杂志》2017年第12期2653-2658,共6页Journal of Clinical and Pathological Research

基　　金：南通大学校级自然科学类科研基金(16ZY36);泰州市人民医院院级课题(ZL201613);2017年泰州市科技支撑计划(社会发展)(SSF20170218)~~

摘　　要：目的:了解泰州市人民医院2016年血培养病原菌的分布和耐药情况。方法:采用BACTEC ALERT3D全自动血培养仪进行血培养;采用VITEK 2 Compact全自动鉴定系统进行微生物鉴定及药敏试验,链球菌药敏为纸片扩散法(K-B法),真菌药敏采用DL-96 FUNGUS;WHONET5.6软件进行统计分析。结果:2016年全年血培养共检出病原菌380株,排列前5位的菌种依次为大肠埃希菌(24.2%)、表皮葡萄球菌(14.7%)、人葡萄球菌(12.1%)、肺炎克雷伯菌(8.7%)、金黄色葡萄球菌(7.9%)。血培养病原菌主要分布于:新生儿ICU(newborn-intensive care unit,NICU)、感染科、ICU、消化科。大肠埃希菌和肺炎克雷伯菌超广谱β-内酰胺酶(extended spectrum β lactamases,ESBLs)检出率分别为53.3%,33.3%。耐甲氧西林金黄色葡萄球菌(methicillin resistant Staphylococcus aureus,MRSA)检出率为44.4%,葡萄球菌未发现对利奈唑胺、万古霉素、奎奴普丁/达福普汀、替加环素耐药的菌株。结论:血培养病原菌在不同科室的分布和耐药性不同。Objective： To investigate the distribution and resistance of pathogens isolated from blood culture of patients in the Jiangsu Taizhou People’s Hospital in 2016. Methods： All blood samples were cultured by BACTEC ALERT 3D. Automatic detection machine of VITEK-2 Compact was used for identification of bacteria and fungus, as well as the susceptibility of non-fastidious bacteria. Susceptibility of streptococcus was tested by K-B method while susceptibility of fungus was tested by DL-96 FUNGUS. WHONET5.6 software was used for statistical analysis. Results： The total of pathogen strains isolated from blood culture in 2016 was 380. The top five bacteria were Escherichia coli （24.2%）, Staphylococcus epidermidis （14.7%）, Staphylococcus hominis （12.1%）, Klebsiella pneumonia （8.7%）, Streptococcus （7.9%）. The pathogens of blood culture were mainly isolated from the department of newborn-intensive care unit （NICU）, infection, ICU, gastroenterology dept. The resistance rates of Escherichia coli and Klebsiella pneumonia to extended spectrumβ lactamases （ESBLs） were 53.3%, 33.3%. Staphylococcus aureus had a 44.4% resistance rate to methicillin, while all of the three aureus were sensitive to linezolid, vancomycin, quinupristin/Dafoe leptin, tigecycline. Conclusion： Diversities of distribution and antimicrobial susceptibility of blood culture isolates are found in different clinical departments.

关 键 词：血培养 病原菌分布 耐药率 

分 类 号：R446.5[医药卫生—诊断学]