脾虚型功能性消化不良大鼠胃组织GRP78/BiP蛋白表达及脾虚1号方干预研究  

Protein Expression of GRP78/BiP in Gastric Tissue of Functional Dyspepsia Rats with Pi Deficiency Syndrome and Intervention Study on Pixu 1 Recipe

作　　者：吕林[1] 王凤云[1] 唐旭东[1] 黄穗平[2] 王静[2] 马祥雪 尹晓岚 石啸双 

机构地区：[1]中国中医科学院西苑医院消化科/北京市中医脾胃病研究所,北京100091 [2]广东省中医院消化科,广州501015 [3]中国中医科学院研究生院,北京100700

出　　处：《中国中西医结合杂志》2018年第1期54-59,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家重点基础研究发展计划(973计划)(No.2013CB531703);国家自然科学基金资助项目(No.81503567);中国博士后科学基金资助项目(No.2015M1227;2016T90195)

摘　　要：目的探讨脾虚型功能性消化不良(FD)大鼠胃组织GRP78/BiP蛋白表达及脾虚1号方的干预作用。方法 70只10日龄雄性SD大鼠随机分为正常组(10只)、FD模型组(10只)、脾虚型FD模型组(50只)。正常组给予2%蔗糖溶液灌胃,0.2 mL/d,连续6天;FD模型组、脾虚型FD模型组给予0.1%蔗糖碘乙酰胺溶液灌胃,0.2 mL/d,连续6天。脾虚型FD模型组正常饲料喂养至6周龄后叠加改良小平台法,连续14天。造模结束后随机分为脾虚型FD模型组,西药组,中药低、中、高剂量组,每组各10只,连同正常组、FD模型组,每天分别给予蒸馏水1 mL/100 g、多潘立酮0.3125 mg/100 g、脾虚1号方0.1275、0.255、0.51 g/100g,灌胃14天。采用Western blot和免疫组化方法检测胃窦组织GRP78/BiP蛋白表达量。结果与正常组比较,脾虚型FD模型组大鼠胃窦黏膜GRP78/BiP蛋白光密度值和蛋白灰度值均升高(P<0.05,P<0.01);与脾虚型FD模型组比较,中药低剂量组大鼠胃窦黏膜GRP78/BiP蛋白表达量降低,而中药中剂量组大鼠胃窦组织GRP78/BiP蛋白灰度值降低(P<0.05,P<0.01)。结论脾虚型FD大鼠胃窦GRP78/BiP蛋白表达升高,存在内质网应激现象,脾虚1号方能够减少GRP78/BiP蛋白的表达,减轻内质网应激现象的发生。Objective To observe the protein expression of GRP78/BiP in gastric tissue of func- tional dyspepsia （FD） rats with Pi deficiency syndrome （PSD） and the intervention by Pixu 1 Recipe （PX1R）. Methods Totally 70 ten-day-old male SD rat pups were randomly divided into the normal control group （n =10）, the FD model group （n =10）, the FD PSD model group （n =50）. Rats in the normal control group were administered 2% sucrose solution （0.2 mL per day） by gastrogavage. Rats in the FD model group and the FD PSD model group were administered with 0.1% Iodoacetamide sucrose solution （0.2 mL per day） by gastrogavage. The medication lasted for all for 6 successive days. Rats in the FD PSD model group were normally fed till they were 6 weeks old, and then they received modified multiple platform method （MMPM） for 14 successive day. After modeling rats were randomly divided into FD PDS model group,Western medicine （WM） group, low, mid, and high dose PX1R group, 10 in each group. Rats in the normal group were administered with 1 mL·100 g -1·d -1 distilled wate by gastrogavage. Those in the VVM group were administered with 0. 3125g·100 g-1·d-1 Domperidone by gastrogavage. Those in low, mid, and high dose PX1R groups were administered by gastrogavage with 0.1275 g·100 g-1·d-1, 0.255g·100 g-1·d-1, 0.51g·100 g-1·d-1 PXlR, respectively. All medication lasted for 14 days. GRP78/BiP protein expressions in gastric antrum tissues were detected by Western blot and immunohistochemistry. Results Compared with the normal group, the optic value and gray value of GRP78/BiP protein increased in the FD PSD model group （P 〈0.05,P 〈0.01 ）. Compared with the FD PSD model group, the amount of GRP78/ BiP protein expressions decreased in the low dose PX1R group. But as compared with the FD PSD model group, the gray value of GRP78/BiP protein decreased in the mid dose PX1R group （P 〈0.05,P 〈0.01 ）. Conclusions GRP78/BiP protein expression of gastric antrum tissue increased in FD PSD model

关 键 词：内质网应激 葡萄糖调节蛋白78 免疫球蛋白重链结合蛋白 功能性消化不良 脾虚证 脾虚1号方 

分 类 号：R285.5[医药卫生—中药学]