UCMSC对放射伤树鼩淋巴细胞表达Th1/Th2类细胞因子的干预作用  

Intervention effect of UCMSCs on expression of Th1/Th2 cytokines in lymphocytes of tree shews with radiation injury

作　　者：郭德镔 李青青[1,3] 王金祥[1] 刘菊芬[1] 王强[1] 杨建勇[1] 朱向情[1] 潘兴华[1] 杨嫒[4] 

机构地区：[1]成都军区昆明总医院细胞生物治疗中心,干细胞与免疫细胞生物医药技术国家地方联合工程实验室,昆明650032 [2]第三军医大学昆明总医院临床学院细胞生物治疗中心 [3]昆明医科大学昆明总医院临床学院细胞生物治疗中心 [4]解放军421医院医保办

出　　处：《西南国防医药》2018年第1期10-12,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：国家科技支撑计划项目(2014BI01B0);昆明市重点项目(2015-1-S-00973);云南省战略性新兴产业专项(2013DA004);全军实验动物专项(SYDW[2014]003)

摘　　要：目的观察脐带间充质干细胞(UCMSC)治疗对放射伤树鼩外周血和脾淋巴细胞表达Th1/Th2类细胞因子的影响。方法成年雄性树鼩随机分成模型组、治疗组,每组15只,两组均接受全身一次性4.5 Gy电离辐照,然后治疗组经股静脉输注同种异体UCMSC(3×10~7个/kg),而模型组则给予同体积生理盐水,均1次/w,连续治疗4次。末次MSC治疗后1 w,统计两组的存活率,实时荧光定量PCR检测两组外周血和脾淋巴细胞IL-2、IL-4、IL-6、TNF-α的m RNA表达水平。结果在末次治疗后1 w,治疗组存活率为86.677%,模型组存活率为53.33%,两组比较有显著差异(P﹤0.05);治疗组外周血和脾淋巴细胞TNF-α、IL-2表达量均低于模型组(P﹤0.05),而IL-4、IL-6表达量均高于模型组(P﹤0.05)。结论 UCMSC治疗可提高全身放射损伤树鼩的生存率,诱导淋巴细胞Th1/Th2类细胞因子表达向Th2优势化。Objective To observe the impact of umbilical cord-derived mesenchymal stem cells （UCMSCs） treatment on the expression of Th1/Th2 cytokines in peripheral and splenic lymphocytes of tree shews with radiation injury. Methods Adult male tree shrews were randomly divided into a model group and a treatment group （n=15, respectively）; both groups received one-time ionizing radiation of 4.5 Gy to the whole body; and then, the treatment group received an infusion of allogeneic UCMSCs （3×10^7 cells/kg） via the femoral vein and the model group was treated with normal saline of the same volume, and both groups were treated for four consecutive times, once per week. One week after the last MSC treatment, the statistical treatment was done on the survival rate of both groups, and then the real-time quantitative polymerase chain reaction （PCR） technique was performed on the peripheral and splenic lymphocytes in both groups to obtain the mRNA expression levels of IL-2, IL-4, IL-6 and TNF-α. Results One week after the last treatment, the survival rate of the treatment group was 86.677% and that of the model group was 53.33%, presenting a significant difference （P 〈 0.05）; the expression levels of TNF-α and IL-2 in the peripheral and splenic cells of the treatment group were lower than those in the model group （P 〈 0.05）, while the expression levels of IL-4 and IL-6 were higher than those in the model group （P 〈 0.05）. Conclusioa The UCMSCs treatment can improve the survival rate of tree shrews with radiation injury to the whole body and induce a switch of the Th1/Th2 cytokine expression in lymphocytes to the dominant Th2 cytokine expression.

关 键 词：树鼩 放射损伤 脐带间充质干细胞 TH1/TH2 细胞因子 

分 类 号：R318[医药卫生—生物医学工程]