BMSC治疗ApoE-/-动脉粥样硬化小鼠模型的初步研究  

A preliminary study on effect of BMSC on atherosclerosis in ApoE^(-/-) mouse model

作　　者：白盈盈[1] 刘菊芬[1] 张建平 和法莲 陈慧芬[1] 张梦圆 王颖翠 潘兴华[1] 刘高米洋[1] 

机构地区：[1]成都军区昆明总医院细胞生物治疗中心、干细胞与免疫细胞生物医药技术国家地方联合工程实验室、云南省细胞治疗技术转化医学重点实验室、云南省干细胞重点实验室,成都军区昆明总医院骨科,昆明医科大学昆明总医院临床学院,昆明650032

出　　处：《西南国防医药》2018年第1期13-15,共3页Medical Journal of National Defending Forces in Southwest China

基　　金：全军实验动物专项面上项目(SYDW2016004);云南省科技创新团队建设项目(20140810)

摘　　要：目的研究骨髓间充质干细胞(BMSC)对ApoE^(-/-)缺陷小鼠动脉粥样硬化(AS)的治疗作用。方法 ApoE^(-/-)缺陷小鼠随机分为空白对照组、阴性对照组和实验组,同时设置正常对照组。实验组、阴性对照组于9 w龄时开始分别经尾静脉注射BMSC悬液200μl(1×10~5个BMSC)和同体积生理盐水,正常对照组和空白对照组不做处理。小鼠分别于12 w时和BMSC注射后4 w,采用全自动生化分析仪测定各组血液总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平,油红O染色观察主动脉弓斑块形成情况。结果空白对照组与正常对照组小鼠相比,TC、TG、LDL-C明显升高(P<0.05),主动脉弓油红O染色可见空白对照组主动脉弓出现斑块。BMSC治疗后,实验组的TC、TG、LDL-C较阴性对照组明显下降(P<0.05),油红染色发现实验组主动脉弓斑块斑块数量及面积均减少。结论 ApoE^(-/-)缺陷小鼠高脂饮食至12 w龄时出现典型动脉粥样硬化(AS)临床表征,而同种异体BMSC具有一定降低血脂、抑制主动脉弓内斑块形成的作用。Objective To study the therapeutic effect of bone marrow-derived mesenchymal stem cells （BMSCs） on the atherosclerosis （AS） in ApoE--- mice. Methodg ApoE-/- mice were randomly divided into a blank control group, a negative control group, an experimental group and a normal control group. From the ninth week, the experimental group and the negative control group were injected with 200 μl （1×10^5 cells） BMSC suspension via caudal vein and normal saline of same volume respectively, while the normal control group and blank control group were not treated. In the 12th week and after four weeks since the BMSC injection, the levels of total cholesterol （TC）, triglyceride （TG）, high density lipoprotein cholesterol （HDL-C） and low density lipoprotein cholesterol （LDL-C） in the blood of all groups were measured by an automatic biochemical analyzer, and the oil red O staining was conducted to observe the formation of plaques in the aortic arch. Methods As compared with the normal control group, the levels of TC, TG and LDL-C in the blank control group increased significantly（P 〈 0.05）; the oil red O staining showed the presence of plaques in the aortic arch of the blank control group. After BMSC treatment, the levels of TC, TG and LDL-C in the experimental group were much lower than those in the negative control group （P 〈 0.05）; the oil red O staining suggested less and smaller plaques in the aortic arch of experimental group. Conclusion ApoE -/- mice fed a high fat diet till the 12th week showed clinical symptoms of typical atherosclerosis （AS）, and the allogeneic BMSCs play a role in reducing blood lipids and inhibiting the formation of plaques in the aortic arch.

关 键 词：间充质干细胞 动脉粥样硬化 ApoE-/-缺陷小鼠 血脂 斑块 

分 类 号：R318[医药卫生—生物医学工程]