脐带间充质干细胞对CD4＋和CD25＋Treg细胞的调节作用  

Regulatory effect of umbilical cord-derived mesenchymal stem cells on CD4^+CD25^+Treg cells

作　　者：王强[1] 何洁[1] 赵晶[1] 石琳琳[1] 马丽花[1] 庞荣清[1] 潘兴华[1] 

机构地区：[1]成都军区昆明总医院细胞生物治疗中心,干细胞与免疫细胞生物医药技术国家地方联合工程实验室,云南省细胞治疗技术转化医学重点实验室,云南省干细胞工程实验室,昆明市干细胞与再生医学研究重点实验室,昆明650032

出　　处：《西南国防医药》2018年第1期22-25,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：云南省科技计划重点项目(2013CA005,2013DA004);成都军区“十二五”医学研究重点项目(B12021)

摘　　要：目的观察人脐带间充质干细胞(UCMSC)对体外CD4^+和CD25^+Treg淋巴细胞的影响。方法分离培养人UCMSC和淋巴细胞,将UCMSC和淋巴细胞按不同比例分别以接触和非接触方式共培养72 h,同时设淋巴细胞单独培养为对照组。收集各组淋巴细胞后,采用流式细胞术检测CD4^+和CD25^+Treg细胞的百分比,并取各组上清液,采用ELISA法检测IL-10含量。结果当淋巴细胞与UCMSC以10∶1接触和非接触共培养时,Treg细胞比例最高,各共培养组CD4^+和CD25^+Treg细胞和IL-10含量显著高于对照组(P<0.05)。结论 UCMSC可以诱导淋巴细胞向Treg细胞的分化,其作用机制可能与UCMSC分泌的细胞因子有关。Objective To observe the effect of umbilical cord-derived mesenchymal stem cells （UCMSCs） on CD4＋ CD25＋ Treg cells in vitro. Methyls Human UCMSCs and lymphocytes were isolated and co-cultured in different proportions in contact and non- contact modes for 72 h; additionally, other lymphocytes were isolated and cultivated to be a control group. Lymphocytes of all groups were collected and treated with the flow cytometry to measure the percentage of CD4＋ CD25＋Treg cells; and then, the supernate of all groups was obtained and treated with the enzyme-linked immunosorbent assay （ELISA） method to measure the level of IL-10. Results When lymphocytes were co-cultured in contact and non-contact modes with UCMSCs in the proportion of 10：1, Treg cells accounted for the highest percentage among all ceils; the levels of CD4＋ CD25＋Treg ceils and IL-10 in all co-culture groups were significantly higher than those in the control group （P 〈 0.05）. Conclusion UCMSCs could induce lymphocytes to differentiate into Treg cells; the mechanism of such differentiation may be related to cytokines released by UCMSCs.

关 键 词：脐带间充质干细胞 CD4＊CD25+Treg细胞 白细胞介素10 

分 类 号：R318[医药卫生—生物医学工程]