空心莲子草总黄酮对HepG2.2.15细胞HBsAg和HBeAg表达的影响  

Effects of flavonoids extracted from Alternanthera philoxeroides on HBsAg and HBeAg expression content in HepG2.2.15 cells

作　　者：武谦虎 徐卫东[2] 李伟东[3] 徐璐 凌美 吴玉华 

机构地区：[1]江苏大学附属镇江三院药物临床试验机构办公室,江苏镇江212000 [2]江苏大学药学院,江苏镇江212013 [3]南京中医药大学药学院,江苏南京210023

出　　处：《中国医药导报》2018年第1期34-37,共4页China Medical Herald

基　　金：江苏省镇江市科技支撑-社会发展项目(SH2012048)

摘　　要：目的研究空心莲子草总黄酮对乙肝病毒的体外抑制作用。方法将HepG2.2.15细胞接种于细胞培养板,然后随机分为阴性对照组、实验组和阳性对照组。阴性对照组用100 mL/L DMSO处理;各实验组分别加入800、400、200、100 mg/L的空心莲子草总黄酮,阳性对照组用800、400、200、100 mg/L的拉米夫定共培养;应用细胞培养技术和光密度测定法研究空心莲子草总黄酮对HepG2.2.15细胞系HBsAg与HBeAg表达的影响。结果各实验组对HepG2.2.15细胞中HBsAg的抑制率均有明显的升高,实验组与阴性对照组比较,差异有统计学意义(P<0.01),实验组与阳性对照组比较,差异有统计学意义(P<0.05),实验组中HBsAg的抑制率跟药物剂量呈正相关;实验组各剂量对HepG2.2.15细胞中HBeAg的抑制率均有明显的升高,实验组与阴性对照组比较,差异有统计学意义(P<0.01),实验组与阳性对照组比较,差异有统计学意义(P<0.05),实验组中HBeAg抑制率跟药物剂量呈正相关。结论空心莲子草总黄酮对HepG2.2.15细胞中HBsAg和HBeAg表达均有明显的抑制作用。体外细胞培养证实空心莲子草总黄酮有较强的抗乙肝病毒作用。Objective To study the anti-HBV action of total flavonoids extracted from Ahernanthera philoxeroides in vitro. Methods HepG2.2.15 cells were inoculated into the cell culture plates and then randomly divided into negative control group, experimental group and positive control group. The negative control group was treated with 100 mL/L DMSO, the experimental groups were treated with 800, 400, 200 and 100 mg/L total flavonoids extracted from Ahernanthera philoxeroides. In the positive control groups, lamivudine was added at the concentration of 800, 400, 200 and 100 mg/L. Cell culture and optical density assay were used to study the effects of total flavonoids from Ahernanthera phiioxeroides on the expression of HBsAg and HBeAg in HepG2.2.15 cell line. Results The inhibit the expression of HBsAg in HepG2.2.15 cells in each experimental group was obviously higher, compared with the negative group, the difference was statistically significant （P 〈 0.01） and compared with the positive control group, the difference was sta- tistically significant （P 〈 0.05）, the inhibition rate of HBsAg in the experimental group was positively correlated with the dose of the drug. Each dose of the experimental groups had obvious inhibitory effects on the expressions of HBeAg in HepG2.2.15 cells, compared with the negative control group, the difference was statistically significant （P 〈 0.01） compared with and positive control group, the difference was statistically significant （P 〈 0.05）, the inhibition rate of HBeAg in the experimental group was positively correlated with the dose of the drug. Conclusion Total flavonoids extracted from Ahernanthera philoxeroides has obvious inhibitory effects on the expressions of HBsAg and HBeAg in HepG2. 2. 15 cells. Total flavonoids extracted from Alternanthera philoxeroides has strong anti-HBV action in vitro.

关 键 词：空心莲子草 总黄酮 HEPG2.2.15细胞 HBSAG HBEAG 

分 类 号：R969[医药卫生—药理学]