应用高通量测序技术对蜱携带RNA病毒的检测研究  

Investigation of RNA viruses in ticks via high-throughput sequencing

作　　者：王慧煜[1] 何鲖鲖 刘佳佳 范航[2] 林祥梅[1] 梅琳[1] 童贻刚[2] 韩雪清[1] 

机构地区：[1]中国检验检疫科学研究院,北京100176 [2]军事医学科学院微生物与流行病学研究所,北京100071

出　　处：《中国兽医科学》2018年第1期48-52,共5页Chinese Veterinary Science

基　　金：“十二五”国家科技支撑计划项目(2013BAD12B03)

摘　　要：利用高通量测序技术,对来源于不同边境地区的蜱类进行宏基因组测序,对所携带的病原基因序列进行鉴定和分类。不经事先筛选,直接对含有未知病原体的蜱类样本提取核酸并进行高通量测序,对测序得到的数据采用与病原体数据库比对的方法进行潜在病原体筛查。结果显示,在4组样本中,有3组样本发现一种或一种以上的可疑病毒,且在2个样本中检测到致病性的发热伴血小板减少综合征病毒（SFTS病毒）及白蛉病毒。结果表明,高通量测序技术可以初步描绘蜱类所携带病毒的多样性,为口岸虫媒携带病原体快速筛查提供依据。The studys aims to screen and identify the viruses carried by ticks in different border areas by highthroughput sequencing technology. We performed high-throughput sequencing of tick RNAand then screening forRNA virus sequences using the sequenced data. In the four samples,three samples were found to contain one or more suspicious viruses,and two samples were found to contain severe fever with thrombocytopenia syndrome virus（SFTS）and phlebovirus virus. High-throughput sequencing technologycan be used to describe the diversity of virus carried by ticks,and provide the basis for rapid pathogen scree- ning in frontier port quarantine.

关 键 词：高通量测序 蜱 RNA病毒 筛查 

分 类 号：S852.746[农业科学—基础兽医学]