对比剂对糖尿病大鼠肾近球小管Na^+,K^+-ATPase活性的影响  

The Effects of Contrast Agent on the Activity of Na^+,K^+-ATPase in Renal Proximal Tubules of Diabetic Rats

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作  者:李冀 陈曦[2] 石理[2] 

机构地区:[1]贵州医科大学第二附属医院心内科,贵州凯里556000 [2]暨南大学第三附属医院心内科,广东珠海519000

出  处:《贵阳中医学院学报》2018年第1期33-37,共5页Journal of Guiyang University of Chinese Medicine

摘  要:目的:就对比剂影响糖尿病大鼠单肾近球小管钾钠离子ATP酶(K^+,Na+-ATPase)活性状况加以探讨;在对比剂急性肾损伤方面,对健康大鼠与糖尿病大鼠展开对比分析;就对比剂肾病发病机理展开研究。方法:对正常成年SD大鼠及造模成功的糖尿病大鼠经尾静脉注射等量低渗对比剂和生理盐水,经过3d取材;通过微分离技术,借助显微镜找到检测部位单肾近球小管,借助电镜完成鉴定,应用液闪法对K^+,Na+-ATPase活性加以测定。结果:与盐水对照组相比,就检测部位的K^+,Na+-ATPase活性而言,对比剂组大鼠单根呈极显著较低表现(P<0.01);与正常大鼠相比,糖尿病大鼠Na+,K^+-ATPase活性明显进一步降低(P<0.01)。结论:就大鼠肾近球小管K^+,Na+-ATPase活性而言,对比剂呈现出一定的抑制作用,其中糖尿病大鼠作用更为显著。Objective:To discuss the effects of contrast agent on the activity dium ions ( K+, Na+-ATPase) ATP enzyme of renal proximal tubule of diabetic of renal potassium ions and sorats; to perform a comparative analysis of both healthy rats and diabetic rat in acute kidney injury of the contrast agent, and to study the nephropathy pathogenesis of the contrast agent. Methods : Gave injection of the same amount of hypotonic contrast agent and normal saline to normal adult SD rats and modeled diabetic rats via tail vein. Determined the activity of K+, Na+-ATPase by liquid scintillation method and microseparation technology. Meanwhile, the renal proxireal tubule in the examined part was located with microscope and identified with electron microscope. Results: Compared with the saline control group, in terms of the activity of K+ , Na+-ATPase in the examined parts, the single root of rats was significantly lower than that of the contrast agent group (P〈0. 01). Compared with the normal rats, the activity of K+ , Na+-ATPase of the diabettic rats decreased significantly. Conclusions : In terms of the activity of K+ and Na+-ATPase in renal proximal tubule, the contrast agent has certain effect of inhibition, especially to diabetic rats. Key words: Contrast agent; acute kidney injury of contrast agent; Sodium potassium ATP enzyme;Diabetic Rat

关 键 词:对比剂 对比剂急性肾损害 钠钾ATP酶 糖尿病大鼠 

分 类 号:R692[医药卫生—泌尿科学]

 

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