产非达霉素药用植物内生放线菌N12W0304的分类鉴定  

Taxonomic studies on a fidaxomicin-producing strain N12W0304 isolated from the medicinal plant

作　　者：丁彦博[1] 穆云龙[1] 霍培元[1] 单越琦[1] 徐岩[1] 路新华[1] 张雪霞[1] 

机构地区：[1]微生物药物国家工程研究中心河北省工业微生物代谢工程技术研究中心华北制药集团新药研究开发有限责任公司,石家庄050015

出　　处：《中国抗生素杂志》2018年第1期40-45,共6页Chinese Journal of Antibiotics

基　　金：国家重大新药创制项目(No.2014ZX09201001-003和No.2012ZX09301002-003);国家自然科学基金项目(No.U1403101)

摘　　要：目的对一株分离自药用植物仙鹤草中的产非达霉素菌株N12W0304进行分类研究。方法通过形态特征、培养特征观察、生理生化特征、胞壁分析及16S r DNA序列分析等多相分类研究对菌株进行鉴定。结果菌株N12W0304属于游动放线菌属(Actinoplanes sp.)菌株。结论本报道是首次从仙鹤草中分离到游动放线菌属非达霉素产生菌。Objective Taxonomic studies on a fidaxomicin-producing strain N12W0304 which was isolated from the medicinal plant Agrimonia pilosa Ledeb. Methods The strain N12W0304 was identified based on morphological, physiological and biochemical characteristics, cell wall components, as well as the sequence analysis of 16S rDNA. Results The polyphasic taxonomical studies showed that the strain N12W0304 belonged to Actinoplanes sp. Conclusion This is the first report of a fidaxomicin-producing strain isolated from Agrimonia pilosa Ledeb.

关 键 词：非达霉素 仙鹤草 内生放线菌 N12W0304 分类鉴定 游动放线菌属 

分 类 号：Q939.13[生物学—微生物学]